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Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (3): 394-400.doi: 10.7525/j.issn.1673-5102.2020.03.010

• Research Report • Previous Articles     Next Articles

Drought Resistance of Larch NAC Gene by Transient Genetic Transformation

ZHANG Lei, XIONG Huan-Huan, CAO Qing, ZHAO Jia-Li, ZHANG Han-Guo   

  1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040
  • Received:2019-08-14 Online:2020-05-05 Published:2020-05-29
  • Supported by:
    National Natural Science Foundation of China(3170059);National Science and Techology Major Project of the Ministry of Science and Technology of China(2018ZX08020-003);Fundamental Research Funds for the Central Universities(2572019BA13)

Abstract: An NAC transcription factor named LoNAC18 was cloned by transcriptome analysis of Larix olgensis. The sequence analysis results showed that the whole gene was 1 101 bp, encoding 366 amino acids, and it was an unstable hydrophilic protein. Compared with the control, gene expression increased of the transient genetic transformation plant under PEG simulated drought stress, while SOD, POD, soluble protein, soluble sugar content was all increased, and the MDA content is lower than the control plants. Therefore, LoNAC18 genes was involved in regulating the L.olgensis response to PEG stress, the results could provide the reference to research on NAC function and mechanism of action of conifer stress response.

Key words: Larix olgensis, transient genetic transformation, NAC, PEG

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