Transcriptome Analysis for Rare and Endangered Plants of Semiliquidambar cathayensis

doi:10.7525/j.issn.1673-5102.2019.02.014

Abstract

Abstract: In order to strengthen the conservation and utilization of China's endangered plant, Semiliquidambar cathayensis, a high-throughput sequencing platform, Illumina HiSeq 2500, was used to sequence the transcriptome of the plants. The obtained data was filtered and assembled by de novo. Then, 77 629 unigenes were obtained through classification and redundancy removal. By comparison analysis, annotation of the 9 functional databases, 45 293 unigenes get annotation information, which was divided into 25 sub-categories by function in KOG with 25 253 functional annotation information obtained. The functional annotation of GO could be divided into such three parts as cell components, biological processes, and molecular functions, which is composed of 2 226, and 17 subclasses respectively (65 subclasses in total). With the comparison of KEGG database, 286 metabolic pathways are found, among which there are 177 pathways to biosynthesis of secondary metabolites, which may be related to the medicinal active components of S.cathayensis. Based on the assembling result, 1 547 unigenes encoding the transcription factors are predicted in 88 gene families. Thus, a family of transcription factors controlling pharmacokinetic synthesis are discovered. Based on the annotation results of S.cathayensis, 12 579 SNP polymorphic sites are detected and 57 671 CDS sites are predicted. This study is the first time to analyze the transcriptional sequences of S.cathayensis, providing a basic data for the in-depth study on molecular biology in S.cathayensis.

Key words: Semiliquidambar cathayensis, transcriptome analysis, gene annotation, medicinal active ingredient, metabolic pathways

CLC Number:

Q949.751.4

YE Xing-Zhuang, LIU Dan, LUO Jia-Jia, FAN Hui-Hua, ZHANG Guo-Fang, LIU Bao, CHEN Shi-Pin. Transcriptome Analysis for Rare and Endangered Plants of Semiliquidambar cathayensis[J]. Bulletin of Botanical Research, 2019, 39(2): 276-286.

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