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Bulletin of Botanical Research ›› 2017, Vol. 37 ›› Issue (3): 424-431.doi: 10.7525/j.issn.1673-5102.2017.03.013

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Expression Analysis of Poplar MYB Transcription Factor Gene Family in Response to Salt Stress

LI Xi-Yan, ZHAO Kai, ZHANG Xue-Mei, ZHOU Bo-Ru   

  1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040
  • Received:2017-02-16 Online:2017-05-15 Published:2017-06-03
  • Supported by:
    National 863 project funding(2013AA102701)

Abstract: MYB transcription factor family is one of the most important transcription factors in plants, and plays an important role in the process of plant secondary metabolism regulation, signal transduction and stress resistance. According to the difference of domain structure of MYB transcription factor, it can be divided into 4 subfamilies:1R-MYB(MYB-relaed), R2R3-MYB, 3R-MYB and 4R-MYB. Among them, the R2R3-MYB sub family with the largest number of members can be further divided into 22 subgroups. Using bioinformatics, the characterizations of conserved domain of Poplar MYB transcription factors, genomic location, amino acid composition and physicochemical properties were analyzed. According to the Arabidopsis MYB transcription factor, the functions of Poplar MYB transcription factor were predicted. Based on the transcriptome sequencing and RT-qPCR, 69 salt-stress responding genes(P ≤ 0.05) were screened from 301 MYB genes, including 32 up-regulated genes and 37 down regulated genes. The study could provide a reference for further study on the function of MYB family genes in Poplar.

Key words: Poplar, MYB transcription factors, bioinformatics, RT-qPCR

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