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Bulletin of Botanical Research ›› 2016, Vol. 36 ›› Issue (1): 75-83.doi: 10.7525/j.issn.1673-5102.2016.01.011

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Transcriptome Analysis for Pinus yunnanensis Based on High Throughput Sequencing

CAI Nian-Hui1,2,3;DENG Li-Li1,2,3;XU Yu-Lan1,2,3;XU Yang1,2,3;ZHOU Li1,2,3;WANG Da-Wei1,2,3;TIAN Bin1,2,3;HE Cheng-Zhong1,2,3;DUAN An-An1,2,3   

  1. 1.Southwest Forestry University,Key Laboratory for Forest Genetic and Tree Improvement & Propagation in Universities of Yunnan Province,Kunming 650224;
    2.Key Laboratory of Biodiversity Conservation in Southwest China,State Forestry Administration,Southwest Forestry University,Kunming 650204;
    3.Key laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China,Ministry of Education,Southwest Forestry University,Kunming 650224
  • Online:2016-01-15 Published:2016-05-18

Abstract: The transcriptome of Pinus yunnanensis was sequenced by using Illumina Hiseq 2 000. In total 80 000 Unigene with an average length of 890 nt and N50 of 1 881 nt were obtained by de novo assembly. Of the Unigene, 43 434, 46 415 and 29 418 Unigenes had significant similarity with known data bank in NR, NT and Swiss-Prot, respectively. 14 792 Unigenes were annotated in clusters of orthologous groups of proteins(COG) and assigned to 25 clusters. 26 743 Unigenes were annotated in gene ontology(GO) and grouped into biological processes, cellular components and molecular function three functional categories, 55 sub-categories. The biological processes were most commonly existed. A total of 25 873 Unigenes were divided into 128 Kyoto Encyclopedia of Genes and Genomes(KEGG) pathways whose functions focused on metabolism. We found some Unigenes related to lignin biosynthesis. The sequence data for P.yunnanensis wiill be helpful for the gene discovery and utilization, molecular marker development and genetic improvement in the further research.

Key words: Pinus yunnanensis, transcriptome, Illumina high throughput sequencing technology