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Bulletin of Botanical Research ›› 2013, Vol. 33 ›› Issue (5): 599-604.doi: 10.7525/j.issn.1673-5102.2013.05.017

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Construction of Expression Vector of ptc-miR801 Gene and Function Analysis

YANG Hui-Qin;JIANG Jing;LIU Ming-Ying;QIAO Gui-Rong;JIANG Yan-Cheng;ZHUO Ren-Ying*   

  1. 1.Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Fuyang 311400;2.College of Life Science and Technology,Xinjiang University,Urumqi 830046
  • Received:1900-01-01 Revised:1900-01-01 Online:2013-09-20 Published:2013-09-20
  • Contact: ZHUO Ren-Ying
  • Supported by:

Abstract: ptc-miR801 was identified from P.cathayana by microRNA library sequence, and it had been confirmed that ptc-miR801 was induced by salt stress. The putative targets were NAC-domain protein and Scarecrow-like gene, which were shown to play important roles in response to salt stress. To investigate the function of ptc-miR801, the artificial miR801 plant expression vector was constructed and transformed into Arabidopsis thaliana. Transformed plants were confirmed by PCR and RT-PCR. The results showed that amiRNA transcript level was markedly improved in transgenic plant compared with non-transgenic plants and the overexpression of ptc-miR801 could enhance the salt tolerance of transgenic plants. Our results would help to clarify the molecular mechanism of P.cathayana under salt stress.

Key words: miRNA, expression vector, Arabidopsis thaliana, salt stress

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