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Bulletin of Botanical Research ›› 2009, Vol. 29 ›› Issue (4): 445-452.doi: 10.7525/j.issn.1673-5102.2009.04.011

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Cloning of Hordeum vulgare Yield Related gene HvYrg1 and Construction of Its Plant RNA Interfere Vector

ZHANG Yu-Liang;WANG Xi-Dong;ZHANG Hua;YANG Feng-Shan;YAO Zheng-Pei;SHI Qing-Hua*   

  1. (1.Xinjiang Agricultural University Agronomy Department,Xinjiang Agricultural University Agricultural Biotechnology Key Laboratory,Urumqi830052) (2.Heilongjiang University Life Science Department,Heilongjiang Microbiology Key Laboratory,Harbin150080)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-07-20 Published:2009-07-20
  • Contact: SHI Qing-Hua
  • Supported by:

Abstract: Barley grain is a complex trait controlled by QTL, and RING E3 ubiquitin ligase plays a vital role in grain yield and protein degradation pathway. According to the conservative region of E3 ubiquitin ligase family homologous sequences, such as rice, Arabidopsis, corn, wheat and yeast, one pair of specific primers was designed and used to clone the corresponding gene from barley by RTPCR amplification. The results showed that a full-length cDNA sequence of HvYrg1 gene from Tibetan barley was obtained, which includes a integrity of open reading frame (ORF) 1 275 bp, encoding 424 amino acids (GenBank No. EU333863). Homology comparison showed that HvYrg1 gene shared 86% identity with rice GW2 gene reported in GenBank. Based on plant expression vector pCAMBIA2300-35s-OCS plasmid, we constructed a plant RNAi vector driven by 35 s promoter, namely pCAM-RNAi-HvYrg1 to further investigate its functions on crop yield formation.

Key words: Hordeum vulgare, HvYrg1 gene, cloning, RNA interfere

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