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Bulletin of Botanical Research ›› 2014, Vol. 34 ›› Issue (4): 524-528.doi: 10.7525/j.issn.1673-5102.2014.04.016

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Contamination Control of Underground Buds in Peony Tissue Culture

QU Wen-Jing;LI Qing*;LIU Yan   

  1. School of Landscape Architecture,Beijing Forestry University,National Engineering Research Center for Flowers,Beijing 100083
  • Received:1900-01-01 Revised:1900-01-01 Online:2014-07-20 Published:2014-07-20
  • Contact: LI Qing
  • Supported by:

Abstract: The selection of different peony explants and sterilization methods were studied to suppress the high contamination rate in underground bud tissue culture of peony. The results show that healthy plump underground buds in moderate size should be first considered and different HgCl2 sterilization time should be applied according to the buds’ size, and 5-8 min is advisable. Striping several outer scale layers and leaving at least 2 inside scale layers and choosing the one-step sterilization method could reduce the contamination rate and ensure a thorough sterilization.

Key words: Paeonia lactiflora, underground buds, tissue culture, contamination

CLC Number: