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Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (5): 753-759.doi: 10.7525/j.issn.1673-5102.2021.05.014

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Tissue Culture and Rapid Propagation System of Gentiana rhodantha

Shi-Jun ZHONG, Bi-Dong ZHANG, Shi-Ting LAI, Hua-Min LIU()   

  1. School of Garden and Life Sciences,Chongqing University of Arts and Sciences,Yongchuan 402160
  • Received:2020-04-26 Online:2021-09-20 Published:2021-07-05
  • Contact: Hua-Min LIU E-mail:liuhuanin@126.com
  • About author:ZHONG Shi-Jun(1999—),male,undergraduate,mainly research on the breeding of plants.
  • Supported by:
    College Students’ of Chongqing Innovative Entrepreneurial Training Plan Program;Chongqing province science and technology innovation project(cstc2019jcyj-msxmX0687)


To establish the tissue culture system of Gentiana rhodantha, the proper explant, disinfecting methods, the best medium for seedling proliferation, for rooting and methods for transplanting were studied respectively. The results showed that the stems with node were the best explants, and the survival rate reached 80% with no contamination by disinfecting with 5% sodium hypochlorite for 10 min; the best medium for bud proliferation was: MS+0.1 mg·L-1 IBA+2.5 mg·L-1 ZT with a proliferation coefficient of 8.94, and the best rooting medium was: 1/2MS+0.3 mg·L-1 IBA with a rooting rate of 100%; the best transplant medium was perlite∶turf=1∶1 with a survival rate up to 100%. This study established a rapid propagation system, provided the technical support for the factory seedling and large-scale cultivation of G. rhodantha.

Key words: Gentiana rhodantha, polyploid, tissue culture

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