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Bulletin of Botanical Research ›› 2006, Vol. 26 ›› Issue (5): 583-588.doi: 10.7525/j.issn.1673-5102.2006.05.015

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Cloning,sequence analysis and evolution of gene atpB sugar beet ATP synthase beta subunit

CUI Jie;XU De-Chang;LI Bin-Sheng;YANG Qian;SUN Jing-Han   

  1. (1.Sugar beet and Sugar Industry Institute,Harbin Institute of Technology,Harbin 150086) (2.Forest botanical garden Heilongjiang,Harbin 150040) (3.Department of Bioscience and Technology, Harbin Institute of Technology,Harbin 150001)
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-09-20 Published:2006-09-20
  • Contact: CUI Jie
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Abstract: atpB gene coding ATP synthase beta subunit,it’s a significant gene in photosynthesis. ATP synthase is a key ferment of energetic supersession in organism.It participates in oxidative phosphorylation and photophosphorylation.Based on the highly conservative of plant chloroplast genomesin evolution, the PCR primers were designed and synthesized according to the corresponding sequences in the chloroplast genomes of Nicotiana tobacum, Spinacia and Oryza sativa for amplifying and cloning DNA fragment containing the chloroplast atpB gene (with accessory No.DQ067451 in GenBank respectively) from Beta vulgaris. The sequence analysis results indicated that the length of the determined nucleotide sequence was 2 293 bp,the coding region of atpB gene contained 1 497 bp, which was deduced coding 498 amino acids. Its DNA sequence homologies were 90.92%、95.79%、87.71% and 86.37%, respectively ,with those of Nicotiana tobacumSpinaciaBrassica napus and Oryza sativa, deduced amino acid sequence homologies were 94.58%、97.19%、92.17% and 91.97%. Amino acid phylogenetic tree was constructed.

Key words: sugar beet, chloroplast genome, atpB gene, clone and sequence analysis

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