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Bulletin of Botanical Research ›› 2018, Vol. 38 ›› Issue (4): 518-525.doi: 10.7525/j.issn.1673-5102.2018.04.005

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Sequencing and Alignment Analysis of the Complete Chloroplast Genome of Littledalea tibetica,an Endemic Species from the Qinghai-Tibet Plateau

LIU Yu-Ping1,2,3,4, LÜ Ting1,2,3,4, ZHU Di1,2,3,4, ZHOU Yong-Hui1,2,3,4, LIU Tao1,2,3,4, SU Xu1,2,3,4   

  1. 1. School of Life Science, Qinghai Normal University, Xining 810008;
    2. Key Laboratory of Medicinal Animal and Plant Resources of the Qinghai-Tibet Plateau in Qinghai Province, Xining 810008;
    3. Key Laboratory of Physical Geography and Environmental Process in Qinghai Province, Xining 810008;
    4. Key Laboratory of Education Ministry on Environments and Resources in the Qinghai-Tibet Plateau, Xining 810008
  • Received:2018-01-23 Online:2018-07-15 Published:2018-07-21
  • Supported by:
    Under the auspices of the National Natural Science Foundation of China(41761009,31260052);The National Natural Science Foundation of Qinghai Province(2017-ZJ-904);"High-end Innovative Talents Thousands of People Plan" in Qinghai Province;"135 High-level Personnel Training Project" in Qinghai Province

Abstract: Littledalea tibetica is an alpine perennial species in the grass tribe Bromeae(Poaceae) that is endemic to the Qinghai-Tibet Plateau and adjacent regions. In the present study, we sequenced the complete chloroplast(cp) genome of L.racemosa on the Illumina MiSeq platform, which was obtained based on the reference cp genome of its related species, Lolium perenne. This study the first complete cp sequence for the Bromeae. The total genome size was 136 852 bp in length with 38.5% GC content. It contains a pair of inverted repeat regions(21 503 bp) separated by a large single copy region(80 970 bp) and a small single copy region(12 876 bp). Within the newly sequenced cp genome, we annotated a total of 141 genes, which included 95 protein-coding genes, 38 tRNAs genes, and 8 rRNAs genes. The most of genes occur within the single copy regions. We used the cp genome of L.tibetica and 30 other chloroplast genomes to perform a phylogenetic analysis, indicating that L.tibetica is closely related species in tribe Triticeae of subfamily Pooideae.

Key words: Poaceae, Littledalea, Littledalea tibetica, chloroplast genome, Illumina sequencing, phylogenetic analysis, Qinghai-Tibet Plateau

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