Structure and Variation Analysis of Chloroplast Genomes in Fagacea

doi:10.7525/j.issn.1673-5102.2018.05.016

Abstract

Abstract: We compared the differences in chloroplast genomes between 14 species of 6 genera in Fagaceae using bioinformatics methods, and constructed a phylogenetic tree based on the taxonomic group Corylus heterophylla to reveal the structural characteristics and variation patterns of chloroplast genomes in Fagaceae.The chloroplast genomes of 14 Fagaceae plants were double-stranded circular structure with a size of about 160 kB with a small difference, with a maximum difference of only 1 366 bp. The order of the genes was basically the same, but the number of genes was different. infA, petG, rpl22, ycf1, ycf15 and many other genes were lost in some species. There are mainly 32 protein gene length variation, the reason is the loss of introns, introns or length of coding region change, Castanopsis concinna gene length was quite variable. The 4 IR borders were relatively conservative. However, part of the rps19 gene sequence was inserted into the IR region due to the boundary expansion in 3 species of Castanea henryi, Castanea pumila and C.concinna. The phylogenetic tree constructed with C.heterophyllaas a taxon has higher support rate and better resolution.The chloroplast genome can be used to analyze the phylogenetic problems of the closely related and rapidly evolving species, and would provide the basis for phylogenetic and evolutional studies.

Key words: Fagaceae, chloroplast genome, structure, variation, phylogeny

CLC Number:

S664.4

ZHANG Yan-Tong, HUANG Jian, SONG Ju, LIN Li-Mei, FENG Ruo-Xuan, XING Zhao-Bin. Structure and Variation Analysis of Chloroplast Genomes in Fagacea[J]. Bulletin of Botanical Research, 2018, 38(5): 757-765.

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