Bulletin of Botanical Research ›› 2007, Vol. 27 ›› Issue (4): 465-468.doi: 10.7525/j.issn.1673-5102.2007.04.018
Previous Articles Next Articles
LI Hai-Ying;MA Chun-Quan;YU Bing;GAO Chuan-Jun;ZHANG Shao-Jun;ZHANG Ying;GUO De-Dong
Received:
Revised:
Online:
Published:
Contact:
Supported by:
Abstract: A monosomic addition line in sugar beet, which was designated as M14 was obtained by the wide cross between cultivated sugar beet (Beta vulgaris L.) and Beta corolliflora Zoss. M14 contained the 18 normal chromosomes of sugar beet plus the B. corolliflora chromosome 9. This alien chromosome in M14 had an average transmission frequency of 96.5% through eggs, the reason it had so high transmission frequency was that it had apomixis genes. By mRNA differential display method, the study compared gene expression at the stage of meiosis in florescence between M14 and cultivated sugar beet (Beta vulgaris L.) A2Y which can carry out normal sexual reproduction. The anchored primer were GT15A, GT15G, GT15C,and twenty arbitrary primers were used. By RT-PCR, six positive specific cDNA fragments were obtained. Analysis was performed on NCBI, and comparison the similarity with published sequences was carried out using the BLASTx program. These cDNA fragments were the candidate fragments to clone the apomixis genes.
Key words: apomixis, apomictic lines M14 in sugar beet, mRNA differential display
CLC Number:
 
LI Hai-Ying;MA Chun-Quan;YU Bing;GAO Chuan-Jun;ZHANG Shao-Jun;ZHANG Ying;GUO De-Dong. Extraction cDNA Fragments Specially Expressed in Lines M14 in Sugar Beet by mRNA Differential Display[J]. Bulletin of Botanical Research, 2007, 27(4): 465-468.
0 / / Recommend
Add to citation manager EndNote|Ris|BibTeX
URL: https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2007.04.018
https://bbr.nefu.edu.cn/EN/Y2007/V27/I4/465