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植物研究 ›› 2010, Vol. 30 ›› Issue (2): 253-256.doi: 10.7525/j.issn.1673-5102.2010.02.022

• 论文 • 上一篇    

蓝莓果渣提取物总酚含量及抗氧化活性研究

高畅;程大海*;高欣;赵尔丰   

  1. 哈尔滨市第三中学,哈尔滨 150001
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-03-20 发布日期:2010-03-20
  • 通讯作者: 程大海
  • 基金资助:
     

Total Phenolic Content and Antioxidant Activities of Blueberry Pomace Extracts

GAO Chang;CHENG Da-Hai*;GAO Xin;ZHAO Er-Feng   

  1. Harbin No.3 Middle School,Harbin 150001
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-03-20 Published:2010-03-20
  • Contact: CHENG Da-Hai
  • Supported by:
     

摘要: 研究了蓝莓果渣提取物总酚含量及其抗氧化活性。分别采用水、40%乙醇及纤维素酶辅助乙醇超声提取蓝莓果渣,并用Folin-Ciocalteu试剂对3种提取物的总酚含量进行评估;并采用DPPH清除实验及O—·2清除实验对3种提取物的抗氧化活性进行研究。实验结果表明,纤维素酶辅助超声提取蓝莓果渣的总酚含量最高,可达425.36±15.21 mg GAE·100 g-1 DW,远远高于水提物(169.46±9.75 mg GAE·100 g-1 DW)及醇提物(218.39±12.54 mg GAE·100 g-1 DW)中的总酚含量。且纤维素酶辅助乙醇超声提取物对DPPH的清除能力为2.67±0.13 g Vc·100 g-1 DW,对O—·2的清除能力2.48±0.14 g Vc·100 g-1 DW,明显好于醇提物及水提物抗氧化活性。

关键词: 蓝莓果渣, 纤维素酶, 超声波, 总酚, 抗氧化, 没食子酸, 抗坏血酸

Abstract: Total phenolics and antioxidant activities of blueberry pomace extracts were studied in the present study. Water, 40% ethanol and cellulase-ultrasonic-assisted extraction were used to extract blueberry pomace in this study. Folin-Ciocalteu method was employed for total phenol contents determination. Moreover, the antioxidant activities of aqueous extracts, ethanol extracts and cellulase-ultrasonic-assisted extracts were determined by DPPH free radical scavenging assay and O2 free radical scavenging assay. Results showed that total phenolic content of cellulase-ultrasonic-assisted extracts (425.36±15.2 mg GAE·100 g-1 DW) was much higher than that of aqueous extracts (169.46±9.7 mg GAE·100 g-1 DW) and ethanol extracts (218.39±12.5 mg GAE·100 g-1 DW). The antioxidant activity of cellulase-ultrasonic-assisted extracts in the DPPH radical scavenging and superoxide radical scavenging assay were 2.67±0.13 g Vc·100 g-1 DW and 2.48±0.14 g Vc·100 g-1 DW, respectively. These values were much lower than those of the aqueous extracts and ethanol extracts.

Key words: blueberry pomace, cellulase, ultrasonic, total phenolic, antioxidant activity, GAE, Vc

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