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植物研究 ›› 2020, Vol. 40 ›› Issue (6): 839-845.doi: 10.7525/j.issn.1673-5102.2020.06.006

• 研究报告 • 上一篇    

稀土镧对铁皮石斛不定芽诱导、植株生长及次生代谢产物合成的影响

苗永美, 童元, 方达, 王宇豪   

  1. 安徽科技学院生命与健康科学学院,凤阳 233100
  • 收稿日期:2020-02-28 出版日期:2020-11-20 发布日期:2020-11-04
  • 作者简介:苗永美(1976—),女,博士,副教授,主要从事药用植物代谢调控及成分提取利用研究。
  • 基金资助:
    安徽省自然科学基金(1808085MC78);安徽科技学院稳定人才项目(SKWD201701);安徽省大学生创新训练计划项目(S201910879206)

Effects of Rare Earth Element Lanthanum on Adventitious Buds Induction, Plantlets Growth and Synthesis of Secondary Metabolites of Dendrobium officinale

Yong-Mei MIAO, Yuan Tong, Da Fang, Yu-Hao Wang   

  1. College of Life and Health Science,Anhui Science and Technology University,Fengyang 233100
  • Received:2020-02-28 Online:2020-11-20 Published:2020-11-04
  • About author:Miao Yong-Mei(1976—),female,doctor,associate professor,major in metabolism regulation and component extraction and utilization of medicinal plants.
  • Supported by:
    Natural Science Foundation of Anhui Province(1808085MC78);Stable Talent Project of Anhui Science and Technology University(SKWD201701);Innovation Training Project for College Students of Anhui Province(S201910879206)

摘要:

采用培养基中添加30~150 μmol·L-1 La(NO33的方式,研究La3+对铁皮石斛不定芽诱导、植株生长及次生代谢产物合成的影响。结果表明:70~90 μmol·L-1的La3+对茎段不定芽诱导和促生效果最好;130 μmol·L-1的La3+能显著提高叶绿素含量,植株在含110 μmol·L-1 La3+培养基上生长最好,鲜重增加了15.22倍,显著高于对照,干鲜比为12.05∶100,比对照提高了64.39%;一定浓度的La3+显著提高干品中4种成分含量,110 μmol·L-1 La3+处理下其多糖含量最高,为98.84 mg·g-1,添加90 μmol·L-1时,总黄酮、总酚和联苄含量最高,为4.31,7.56和21.01 mg·g-1。联苄和总黄酮含量与其他5个指标相关性较大,而总酚和叶绿素含量与其他5个指标相关性小。本研究为进一步探究稀土对铁皮石斛促生及改善品质的作用机制奠定基础,为植物组培中如何科学合理使用稀土元素提供理论依据。

关键词: 铁皮石斛, 镧, 离体培养, 多糖, 总黄酮, 总酚, 联苄

Abstract:

The experiment was conducted to study the effects of rare earth element lanthanum on adventitious buds induction, plantlets growth and secondary metabolites synthesis of Dendrobium officinale by adding 30-150 μmol·L-1 La(NO33 to the media. The effects of 70-90 μmol·L-1 La3+ on induction and growth of adventitious buds were the best. The treatment of 130 μmol·L-1 La3+ could significantly increase chlorophyll content of seedlings, and 110 μmol·L-1 La3+ could significantly promote plant growth with 15.22 times of fresh weight, and 12.05∶100 of dry weight-fresh weight ratio, which was 64.39% higher than that of control. A certain concentration of La3 + could significantly improve the content of four components in D.officinale. The polysaccharide content reached the peak value of 98.84 mg·g-1 under 110 μmol·L-1 La3+ treatment. The contents of flavonoids, phenolic acid and bibenzyl reached maximum value of 4.31, 7.56 and 21.01 mg·g-1, respectively, when 90 μmol·L-1 La3+ added to media. Both indexes of bibenzyl and flavonoids were highly correlated with other five indexes, while phenolic acid and chlorophyll content were little correlated with other indexes. This study laid the foundation for further exploring the mechanism of rare earth on promoting growth and improving quality of D.officinale, and provided a theoretical basis for scientific and rational utilization of rare earth elements in plant tissue culture.

Key words: Dendrobium officinale Kimura et Migo, lanthanum, culture in vitro, polysaccharides, total flavonoids, total phenols, bibenzyl

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