杨树ERF11转录因子基因应答渗透胁迫表达分析

doi:10.7525/j.issn.1673-5102.2020.03.015

摘要/Abstract

摘要： 从小黑杨（Populus simonii×P.nigra）叶片中克隆出732 bp的ERF11转录因子基因（Potri.011G057000.1）cDNA，其编码的蛋白含有243个氨基酸，属于不稳定的亲水蛋白，不存在信号肽，不具跨膜能力，含AP2/ERF家族保守结构域。系统进化树表明，小黑杨ERF11蛋白与胡杨、麻疯树、蓖麻、橡胶树、木薯遗传距离较近，说明其亲缘关系较近。亚细胞定位结果表明，ERF11蛋白定位于细胞核中。ERF11基因表达具有组织特异性，并受胁迫诱导表达。其在根中相对表达水平明显比在茎和叶中表达水平高，在盐和甘露醇胁迫下，基因均表现上调表达。表明ERF11转录因子基因可能与植物应答高盐和干旱胁迫相关。

关键词: 小黑杨, 转录因子, 基因表达, 胁迫

Abstract: The ERF11 transcription factor gene(Potri.011G05700.1) cDNA is encoded with 243 amino acids in Populus simonii×P.nigra. ERF11 gene protein is an unstable pro-water protein, has no signal peptide, has no transmembrane ability, and contains AP2/ERF family conservative structure. The systematic evolution tree shows that PsnERF11 protein is close to Populus trichocarpa, Euphratica, Ricinus communis, Hevea brasiliensis, Jatropha curcas, Manihotesculenta, indicating that the genetic relationship is relatively close. The results of subcellular positioning show that ERF11 protein is located in the nucleus. ERF11 gene expression is tissue-specific and induced by coercion. Its relative expression level in the root was significantly higher than in the stem and leaf, and the gene was expressed higher under the pressure of salt and glycol. It is shown that ERF11 transcription factor genes may be associated with plant response to high salt and drought stress.

Key words: Populus simonii×P.nigra, transcription factors, gene expression, stress

中图分类号:

S722.3+6

刘悦, 赵凯, 吕冠斌, 姜廷波, 周博如. 杨树ERF11转录因子基因应答渗透胁迫表达分析[J]. 植物研究, 2020, 40(3): 433-440.

LIU Yue, ZHAO Kai, Lü Guan-Bin, JIANG Ting-Bo, ZHOU Bo-Ru. Transcription Factor Gene ERF11 Response to Osmotic Stress in Populus simonii×P.nigra Poplar[J]. Bulletin of Botanical Research, 2020, 40(3): 433-440.

参考文献

[1] Zhang H,Jin J,Tang L,et al.PlantTFDB 2.0:update and improvement of the comprehensive plant transcription factor database[J].Nucleic acids research,2010,39(suppl_1):D1114-D1117.
[2] Mizoi J,Shinozaki K,Yamaguchi-Shinozaki K.AP2/ERF family transcription factors in plant abiotic stress responses[J].Biochimica et Biophysica Acta(BBA)-Gene Regulatory Mechanisms,2012,1819(2):86-96.
[3] Thirugnanasambantham K,Durairaj S,Saravanan S,et al.Role of ethylene response transcription factor(ERF) and its regulation in response to stress encountered by plants[J].Plant Molecular Biology Reporter,2015,33(3):347-357.
[4] 赵金玲,姚文静,王升级,等.杨树AP2/ERF转录因子家族生物信息学分析[J].东北林业大学学报,2015,43(10):21-29. Zhao J L,Yao W J,Wang S C,et al.AP2/ERF Gene Family in Populus trichocarpa by Bioinformatics[J].Journal of Northeast Forestry University,2015,43(10):21-29.
[5] 闻可心,刘雪梅.AP2功能基因在植物花发育中的重要作用[J].生物技术通报,2010(02):1-7. Wen K X,Liu X M.The Important Role of AP2 Functional Genes in Plant Floral Development[J].Biotechnology Bulletin,2010(02):1-7.
[6] Nakano T,Suzuki K,Fujimura T,et al.Genome-wide analysis of the ERF gene family in Arabidopsis and rice[J].Plant physiology,2006,140(2):411-432.
[7] Lorenzo Oscar,Piqueras Raquel,Sánchez-Serrano Jose J,et al.Ethylene response factor integrates signals from ethylene and jas monate pathways in plant defense[J].The Plant Cell,2003,15(1):165-178.
[8] Xie Z,Nolan T,Jiang H,et al.The AP2/ERF transcription factor TINY modulates brassinosteroid-regulated plant growth and drought responses in Arabidopsis[J].The Plant Cell,2019,31(8):1788-1806.
[9] Karanja B K,Xu L,Wang Y,et al.Genome-wide characterization of the AP2/ERF gene family in radish(Raphanus sativus L.):Unveiling evolution and patterns in response to abiotic stresses[J].Gene,2019,718:144048.
[10] 王雅慧,李彤,黄莹,等.番茄2个ERF-B1亚族转录因子基因的克隆及其对生物和非生物胁迫响应[J].核农学报,2019,33(10):1893-1904. Wang Y H,Li T,Huang Y,et al.Cloning and Expression Analysis Under Biotic and Abiotic Stresses of Two ERF-B1 Group Transcription Factor Genes From Solanum lycopersicum[J].Chinese Journal of Nuclear Agriculture,2019,33(10):1893-1904.
[11] Li Y,Luo C,Chen Y,et al.Transcriptome-based Discovery of AP2/ERF tRanscription Factors and Expression Profiles Under Herbivore Stress Conditions in Bamboo(Bambusa emeiensis)[J].Journal of Plant Biology,2019,62(4):297-306.
[12] 陈韵琳.毛果杨PtrDREB28转录因子功能的研究[D].东北林业大学,2015. Chen Y L.Study on the Function of PtrDREB28 Transcription Factor in Populus trichocarpa[D].Northeast Forestry University,2015.
[13] 姚文静.杨树转录因子ERF76基因耐盐功能研究[D].东北林业大学,2016. Yao W J.Functional Analysis of Poplar Transcription Factor ERF76 Gene on Salt-Stress Tolerance[D].Northeast Forestry University,2016.
[14] Zhou X,Zhang Z L,Park J,et al.The ERF11 transcription factor promotes internode elongation by activating gibberellin biosynthesis and signaling[J].Plant physiology,2016,171(4):2760-2770.
[15] Dubois M,Van den Broeck L,Claeys H,et al.The ETHYLENE RESPONSE FACTORs ERF6 and ERF11 antagonistically regulate mannitol-induced growth inhibition in Arabidopsis[J].Plant physiology,2015,169(1):166-179.
[16] 张文慧.白桦BPERF11基因响应高盐干旱胁迫的调控机理研究[D].东北林业大学,2016. Zhang W H.Regulation in Mechanism of BpERF11 Gene from Betula platyphylla Response to Severe Salt and Drought Stress[D].Northeast Forestry University,2016.
[17] Zheng X,Xing J,Zhang K,et al.Ethylene response factor ERF11 activates BT4 transcription to regulate immunity to Pseudomonas syringae[J].Plant physiology,2019,180(2):1132-1151.
[18] 龚道勇.慈竹bZIP和NRT基因克隆及功能初步研究[D].西南科技大学,2018. Gong Daoyong.Studies on Cloning of BebZIP and BeNRT Genes and Their Preliminary Function in Bambusa emeiensis[D].Southwest University of Science and Technology,2018.
[19] Tuteja N.Abscisic acid and abiotic stress signaling[J].Plant signaling & behavior,2007,2(3):135-138.
[20] 卓维,陈倩,罗银,等.烟草乙烯转录因子ERF基因NtTOE3的克隆、载体构建及表达分析[J].植物研究,2018,38(05):733-740. Zhuo W,Chen Q,Luo Y,et al.Cloning,Construction of Expression Vector and Expression Analysis of the Ethylene-responsive Factor ERF Gene NtTOE3 in Nicotiana tabacum[J].Plant Research,2018,38(05):733-740.
[21] Kasuga M,Liu Q,Miura S,et al.Improving plant drought,salt,and freezing tolerance by gene transfer of a single stress-inducible transcription factor[J].Nature biotechnology,1999,17(3):287.
[22] 王康君,樊继伟,陈凤,等.植物对盐胁迫的响应及耐盐调控的研究进展[J].江西农业学报,2018,30(12):31-40. Wang K J,Fan J W,Chen F,et al.Research Advances in Response of Plants to Salt Stress and Regulation of Salinity Tolerance[J].Jiangxi Agricultural Journal,2018,30(12):31-40.
[23] Zhang J L,Shi H.Physiological and molecular mechanisms of plant salt tolerance[J].Photosynthesis research,2013,115(1):1-22.
[24] Gutterson N,Reuber T L.Regulation of disease resistance pathways by AP2/ERF transcription factors[J].Current opinion in plant biology,2004,7(4):465-471.
[25] Tang W,Charles T M,Newton R J.Overexpression of the pepper transcription factor CaPF1 in transgenic Virginia pine(Pinus virginiana Mill.) confers multiple stress tolerance and enhances organ growth[J].Plant molecular biology,2005,59(4):603-617.
[26] Kizis D,Lumbreras V,Pagès M.Role of AP2/EREBP transcription factors in gene regulation during abiotic stress[J].FEBS letters,2001,498(2-3):187-189.
[27] Sakuma Y,Liu Q,Dubouzet J G,et al.DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs,transcription factors involved in dehydration-and cold-inducible gene expression[J].Biochemical and biophysical research communications,2002,290(3):998-1009.
[28] Cheng M C,Liao P M,Kuo W W,et al.The Arabidopsis ETHYLENE RESPONSE FACTOR1 regulates abiotic stress-responsive gene expression by binding to different cis-acting elements in response to different stress signals[J].Plant physiology,2013,162(3):1566-1582.
[29] Rong W,Qi L,Wang A,et al.The ERF transcription factor Ta ERF 3 promotes tolerance to salt and drought stresses in wheat[J].Plant biotechnology journal,2014,12(4):468-479.
[30] Zhou J,Zhang H,Yang Y,et al.Abscisic acid regulates TSRF1-mediated resistance to Ralstonia solanacearum by modifying the expression of GCC box-containing genes in tobacco[J].Journal of experimental botany,2008,59(3):645-652.
[31] Pan Y,Seymour G B,Lu C,et al.An ethylene response factor(ERF5) promoting adaptation to drought and salt tolerance in tomato[J].Plant cell reports,2012,31(2):349-360.
[32] Li M Y,Liu J X,Hao J N,et al.Genomic identification of AP2/ERF transcription factors and functional characterization of two cold resistance-related AP2/ERF genes in celery(Apium graveolens L.)[J].Planta,2019:1-16.