水稻盐碱逆境响应锌指蛋白基因OsZFP6表达特性及功能研究

doi:10.7525/j.issn.1673-5102.2020.03.014

植物研究 ›› 2020, Vol. 40 ›› Issue (3): 424-432.doi: 10.7525/j.issn.1673-5102.2020.03.014

水稻盐碱逆境响应锌指蛋白基因OsZFP6表达特性及功能研究

刘佳丽¹, 何明良^2,3, 刘晨曦¹, 廖栩¹, 李秀峰^2,3, 管清杰¹

1. 东北盐碱植被恢复与重建教育部重点实验室, 东北林业大学生命科学学院, 哈尔滨 150040;
2. 中国科学院东北地理与农业生态研究所, 哈尔滨 150081;
3. 中国科学院大学, 北京 100049

收稿日期:2019-10-01 出版日期:2020-05-05 发布日期:2020-05-29
通讯作者: 管清杰,E-mail:guanqingjie@nefu.edu.cn E-mail:guanqingjie@nefu.edu.cn
作者简介:刘佳丽(1994-),女,硕士,主要从事生物化学与分子生物学方面的研究。
基金资助:
黑龙江省自然科学基金（C2017009）；国家重点研究开发计划（2016YFC0501203）；中央大学基础研究基金（2572016EAJ6）；黑龙江省博士后科研发展基金（LBH-Q15004）

Zinc Finger Protein OsZFP6 Expression Features and Functions in Saline-alkali Stress Response

LIU Jia-Li¹, HE Ming-Liang^2,3, LIU Chen-Xi¹, LIAO Xu¹, LI Xiu-Feng^2,3, GUAN Qing-Jie¹

1. Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin 150040;
2. Northeast Institute of Geography and Agricultural Ecology of the Chinese Academy of Sciences, Harbin 150081;
3. University of the Chinese Academy of Sciences, Beijing 100049

Received:2019-10-01 Online:2020-05-05 Published:2020-05-29
Supported by:
Natural Science Foundation of Heilongjiang Province of China(C2017009);National Key Research and Development Program of China(2016YFC0501203);Fundamental Research Funds for the Central Universities(2572016EAJ6);Heilongjiang Postdoctoral Scientific Research Developmental Fund(LBH-Q15004)

摘要/Abstract

摘要： 筛选到C2HC型锌指蛋白，其编码304个氨基酸，命名为OsZFP6。分别使用碱性盐（NaHCO₃）和H₂O₂对水稻两周龄幼苗进行胁迫处理，检测到OsZFP6相对表达量受其诱导，并在叶和根中出现差异，因此，推测OsZFP6参与相应的逆境响应调节途径。我们将一个绿色荧光蛋白（GFP）与之融合，发现OsZFP6定位在细胞核。此外，对过表达OsZFP6的水稻转基因植株进行NaHCO₃胁迫处理，结果表明过表达植株均具有较强的耐受性；并且在株高、鲜重生理指标上，野生型植株低于过表达OsZFP6植株，在野生型植株体内的MDA、H₂O₂含量高于过表达植株；说明OsZFP6在响应NaHCO₃胁迫时可以通过清除过氧化物来增强植物的抗性。初步推测OsZFP6基因参与响应碱性盐胁迫途径的调控，可能在转录调控中直接或间接发挥重要作用。

关键词: 锌指蛋白, 非生物胁迫, 水稻, 碱性盐

Abstract: C2HC type zinc finger protein was screened, encoded 304 amino acids and named OsZFP6. The expression level of OsZFP6 was increased by abiotic stress, including alkali(NaHCO₃) and H₂O₂ treatment in two-week old rice leaves but decreased in roots. Thus, it was speculated that OsZFP6 was involved in the regulation corresponding reaction pathways of resistance. We fused a green fluorescent protein(GFP) with it and found that OsZFP6 was located in the nucleus. Moreover, rice overexpressed OsZFP6 were more tolerant to NaHCO₃ treatments. The overexpressed plants had strong tolerance, and were higher than WT in plant height and fresh weight, but the contents of MDA and H₂O₂ in vivo were lower than non-transgenic plants, which indicated that OsZFP6 could enhance the resistance of plants by scavenging peroxide in response to NaHCO₃ stress. Preliminarily, it was speculated that OsZFP6 gene participated in the regulation of resistance and it may play a vital role in transcription regulation.

Key words: zinc finger protein, abiotic stress, rice, basic salt

中图分类号:

S511.5

刘佳丽, 何明良, 刘晨曦, 廖栩, 李秀峰, 管清杰. 水稻盐碱逆境响应锌指蛋白基因OsZFP6表达特性及功能研究[J]. 植物研究, 2020, 40(3): 424-432.

LIU Jia-Li, HE Ming-Liang, LIU Chen-Xi, LIAO Xu, LI Xiu-Feng, GUAN Qing-Jie. Zinc Finger Protein OsZFP6 Expression Features and Functions in Saline-alkali Stress Response[J]. Bulletin of Botanical Research, 2020, 40(3): 424-432.

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水稻盐碱逆境响应锌指蛋白基因OsZFP6表达特性及功能研究

Zinc Finger Protein OsZFP6 Expression Features and Functions in Saline-alkali Stress Response

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