壳斗科植物叶绿体基因组结构及变异分析

doi:10.7525/j.issn.1673-5102.2018.05.016

植物研究 ›› 2018, Vol. 38 ›› Issue (5): 757-765.doi: 10.7525/j.issn.1673-5102.2018.05.016

壳斗科植物叶绿体基因组结构及变异分析

张妍彤¹, 黄剑², 宋菊¹, 林丽梅¹, 冯若宣¹, 邢朝斌¹

1. 华北理工大学生命科学学院, 唐山 063210;
2. 百色学院农业与食品工程学院, 百色 533000

收稿日期:2018-05-03 出版日期:2018-09-15 发布日期:2018-08-31
通讯作者: 邢朝斌 E-mail:xzbheuu@126.com
作者简介:张妍彤(1994-),女,硕士研究生,主要从事生物信息在药用植物中的应用。
基金资助:
国家自然科学基金项目（31760221）；河北省教育厅资助科研项目（QN2014102）；华北理工大学培育基金（SP201508）

Structure and Variation Analysis of Chloroplast Genomes in Fagacea

ZHANG Yan-Tong¹, HUANG Jian², SONG Ju¹, LIN Li-Mei¹, FENG Ruo-Xuan¹, XING Zhao-Bin¹

1. College of Life Sciences, North China University of Science and Technology, Tangshan 063210;
2. Agricultural and Food Engineering, Baise University, Baise 533000

Received:2018-05-03 Online:2018-09-15 Published:2018-08-31
Supported by:
National Natural Science Foundation of China(31760221);Hebei Provincial Department of Education Funding Research Project(QN2014102);Education Fund of North China University of Science and Technology(SP201508)

摘要/Abstract

摘要： 利用生物信息学方法比较壳斗科6个属14个物种的叶绿体基因组间差异，以近缘物种榛为外类群构建系统进化树，揭示壳斗科叶绿体基因组的结构特征及变异规律。结果显示，14种壳斗科植物的叶绿体基因组均为双链环状分子结构，大小在160 kB左右，差异较小，最大仅差1 366 bp；基因顺序基本一致，而基因数量有所差异，infA、petG、rpl22、ycf1、ycf15等多个基因在部分物种中发生丢失；主要有32个蛋白编码基因长度发生变异，其原因是内含子的丢失、内含子或者编码区的长度改变，华南锥基因长度变异较大；4个IR边界相对保守，但锥栗、Castanea pumila、华南锥3个物种由于边界扩张导致rps19基因部分序列进入到IR区；以榛为外类群构建的系统发育树，各进化支支持率较高，分辨率较好。研究结果表明，叶绿体基因组可以用于分析关系较近与进化较快物种的系统发生问题，为系统发育和进化研究提供依据。

关键词: 壳斗科, 叶绿体基因组, 结构, 变异, 系统发育

Abstract: We compared the differences in chloroplast genomes between 14 species of 6 genera in Fagaceae using bioinformatics methods, and constructed a phylogenetic tree based on the taxonomic group Corylus heterophylla to reveal the structural characteristics and variation patterns of chloroplast genomes in Fagaceae.The chloroplast genomes of 14 Fagaceae plants were double-stranded circular structure with a size of about 160 kB with a small difference, with a maximum difference of only 1 366 bp. The order of the genes was basically the same, but the number of genes was different. infA, petG, rpl22, ycf1, ycf15 and many other genes were lost in some species. There are mainly 32 protein gene length variation, the reason is the loss of introns, introns or length of coding region change, Castanopsis concinna gene length was quite variable. The 4 IR borders were relatively conservative. However, part of the rps19 gene sequence was inserted into the IR region due to the boundary expansion in 3 species of Castanea henryi, Castanea pumila and C.concinna. The phylogenetic tree constructed with C.heterophyllaas a taxon has higher support rate and better resolution.The chloroplast genome can be used to analyze the phylogenetic problems of the closely related and rapidly evolving species, and would provide the basis for phylogenetic and evolutional studies.

Key words: Fagaceae, chloroplast genome, structure, variation, phylogeny

中图分类号:

S664.4

张妍彤, 黄剑, 宋菊, 林丽梅, 冯若宣, 邢朝斌. 壳斗科植物叶绿体基因组结构及变异分析[J]. 植物研究, 2018, 38(5): 757-765.

ZHANG Yan-Tong, HUANG Jian, SONG Ju, LIN Li-Mei, FENG Ruo-Xuan, XING Zhao-Bin. Structure and Variation Analysis of Chloroplast Genomes in Fagacea[J]. Bulletin of Botanical Research, 2018, 38(5): 757-765.

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壳斗科植物叶绿体基因组结构及变异分析

Structure and Variation Analysis of Chloroplast Genomes in Fagacea

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