拟南芥转录共激活子ANGUSTIFOLIA3(AN3)调控花的雄蕊的形成

doi:10.7525/j.issn.1673-5102.2018.02.013

植物研究 ›› 2018, Vol. 38 ›› Issue (2): 254-259.doi: 10.7525/j.issn.1673-5102.2018.02.013

拟南芥转录共激活子ANGUSTIFOLIA3(AN3)调控花的雄蕊的形成

李丹, 徐梦珂, 蒋继宏, 孟来生

江苏师范大学江苏省药食植物生物技术国家重点实验室培育点/生命科学学院, 徐州 221116

收稿日期:2017-05-08 出版日期:2018-03-15 发布日期:2018-03-23
通讯作者: 孟来生 E-mail:menglsh@jsnu.edu.cn
作者简介:李丹(1992-),女,硕士研究生,主要从事分子生物学研究。
基金资助:
徐州市农业科技项目（农业高技术攻关项目KC16NG063）

Arabidopsis thaliana Transcription Coactivator ANGUSTIFOLIA3(AN3) in Regulating the Stamen Formation

LI Dan, XU Meng-Ke, JIANG Ji-Hong, MENG Lai-Sheng

The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, College of Life Science, Jiangsu Normal University, Xuzhou 221116

Received:2017-05-08 Online:2018-03-15 Published:2018-03-23
Supported by:
Agricultural science and technology(agricultural high-tech research project)(KC16NG063)

摘要/Abstract

摘要： 雄蕊是种子植物产生花粉的重要生殖器官，其是否正常发育关乎到植物的繁殖状况，并且会对农作物的产量造成影响。通过RT-PCR技术鉴定拟南芥转录共激活子ANGUSTIFOLIA3（AN3）的两个敲除突变体an3-1和an3-4；通过形态学检测发现，突变体an3-1和突变体an3-4的雄蕊较野生型雄蕊短，而雌蕊却无明显变化；通过构建AN3启动子GUS表达载体，对Pro-AN3-GUS植株的花组织进行染色，并观察，结果表明，AN3基因在拟南芥的种子胚、成熟的花粉、柱头、花瓣中均有表达。这个结果证明AN3能在拟南芥生殖生长期间在花器官等重要组织中表达，这个结果与an3-1和an3-4的雄蕊变短的结论一致。由此，我们得出结论：拟南芥转录共激活子AN3正向调控花的雄蕊的形成。

关键词: ANGUSTIFOLIA3(AN3), 突变体, 雄蕊, 调控

Abstract: Stamens are important reproductive organs which produce pollen in seed plants, and their normal development is related to the reproductive status of plants and affects the yield of crops. Two knockout mutants an3-1 and an3-4 of Arabidopsis thaliana transcription coactivator ANGUSTIFOLIA3(AN3) were identified by RT-PCR. By morphological examination, the stamens of the an3-1 and an3-4 mutants were shorter than those of wild-type, whereas, the pistils were not changed significantly. By constructing AN3 promoter with GUS expression vector, the flower tissues of obtained Pro-AN3-GUS plants were stained and observed. The AN3 promoter in Arabidopsis seed embryos, mature pollen, stigma, petals was expressed. AN3 could be expressed in important tissues such as floral organs during Arabidopsis reproductive growth. These above results are consistent with the short stamens of an3-1 and an3-4 mutants. Thus, AN3 positively regulates the stamen development of A.thaliana.

Key words: ANGUSTIFOLIA3(AN3), mutant, stamens, regulation

中图分类号:

Q944.57

李丹, 徐梦珂, 蒋继宏, 孟来生. 拟南芥转录共激活子ANGUSTIFOLIA3(AN3)调控花的雄蕊的形成[J]. 植物研究, 2018, 38(2): 254-259.

LI Dan, XU Meng-Ke, JIANG Ji-Hong, MENG Lai-Sheng. Arabidopsis thaliana Transcription Coactivator ANGUSTIFOLIA3(AN3) in Regulating the Stamen Formation[J]. Bulletin of Botanical Research, 2018, 38(2): 254-259.

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拟南芥转录共激活子ANGUSTIFOLIA3(AN3)调控花的雄蕊的形成

Arabidopsis thaliana Transcription Coactivator ANGUSTIFOLIA3(AN3) in Regulating the Stamen Formation

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