蝴蝶兰PhalPI基因的克隆及在花器官突变体中的表达分析

doi:10.7525/j.issn.1673-5102.2017.03.012

植物研究 ›› 2017, Vol. 37 ›› Issue (3): 416-423.doi: 10.7525/j.issn.1673-5102.2017.03.012

蝴蝶兰PhalPI基因的克隆及在花器官突变体中的表达分析

袁秀云¹, 许申平¹, 王莹博², 崔波¹

1. 郑州师范学院生物工程研究所, 郑州 450044;
2. 河南农业大学生命科学学院, 郑州 450002

收稿日期:2016-11-08 出版日期:2017-05-15 发布日期:2017-06-03
通讯作者: 崔波,E-mail:laocuibo@163.com E-mail:laocuibo@163.com
作者简介:袁秀云(1970-),女,博士,教授,主要从事植物生物技术方面的研究。
基金资助:
河南省科技攻关项目（162102110073）

Cloning of PhalPI Gene from Phalaenopsis and Its Expression in Floral Organ Mutants

YUAN Xiu-Yun¹, XU Shen-Ping¹, WANG Ying-Bo², CUI Bo¹

1. Institute of Bioengineering, Zhengzhou Normal University, Zhengzhou 450044;
2. College of Life Science, Henan Agricultural University, Zhengzhou 450002

Received:2016-11-08 Online:2017-05-15 Published:2017-06-03
Supported by:
Science and technology research projects of Henan Province(162102110073)

摘要/Abstract

摘要： 为深入研究兰科植物花器官发育的调控机理，从蝴蝶兰花瓣中克隆了一个B类MADS-box转录因子PhalPI（GenBank登录号为KY020416）。序列分析表明，该基因的cDNA全长为944 bp，含完整的开放阅读框，可编码210个氨基酸，属于BGLO/PI蛋白家族，与蝴蝶兰属的PhPI10和PeMADS6基因关系最近；表达模式分析表明，PhalPI基因在生殖器官中表达，在营养器官中不表达，在授粉后的子房中，该基因的表达水平降低。在5种花器官突变体中，PhalPI基因在萼片唇瓣化突变体的萼片和蕊柱中表达水平明显升高；在雄蕊花瓣化突变体的萼片和侧瓣中表达水平降低，在其唇瓣和蕊柱中显著升高；在侧瓣合柱化突变体的蕊柱中，PhalPI基因的表达也发生了显著升高；PhalPI基因表达的改变与花器官形态的突变相关；而在侧瓣唇瓣化和侧瓣花药化突变体中，PhalPI基因的表达水平没有变化。推测该基因在决定蝴蝶兰侧瓣和唇瓣的发育中起重要的调控作用。

关键词: 蝴蝶兰, GLO/PI, MADS-box, 花器官突变体

Abstract: For the regulatory mechanism of floral organ development in orchid, PhalPI gene(GenBank accession number:KY020416) was cloned from Phalaenopsis petals. By the sequence analysis, the cDNA full-length of this gene was 944 bp encoding 210 amino acid residues. The gene belongs to the GLO/PI group of the B class MADS-box transcription factor, which was highly identified with PhPI10 and PeMADS6 from Phalaenopsis. The PhalPI was expressed in reproductive organs and not in vegetative organs, the expression level was reduced in ovary after pollination. In 5 floral organ mutants, the expression level of the PhalPI gene was higher significantly in sepal and column of lip-like sepal mutants than that of the normal. The expression of this gene was decreased in sepals and petals in petal-like stamens mutant, and it was increased significantly in lip and column in this mutant. In column of Lateral petals pillared mutants, the expression of PhalPI gene was also increased significantly. The changes of expression level of the PhalPI gene in 3 floral organ mutants were associated with morphological mutations of floral organ. However, the expression levels of PhalPI gene were not changed in lip-like Lateral petals and anther-like Lateral petals mutants. Therefore, the PhalPI gene plays a crucial role in regulating Lateral petal and lip development in Phalaenopsis.

Key words: Phalaenopsis, GLO/PI, MADS-box, floral organ mutants

中图分类号:

S682.31

袁秀云, 许申平, 王莹博, 崔波. 蝴蝶兰PhalPI基因的克隆及在花器官突变体中的表达分析[J]. 植物研究, 2017, 37(3): 416-423.

YUAN Xiu-Yun, XU Shen-Ping, WANG Ying-Bo, CUI Bo. Cloning of PhalPI Gene from Phalaenopsis and Its Expression in Floral Organ Mutants[J]. Bulletin of Botanical Research, 2017, 37(3): 416-423.

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蝴蝶兰PhalPI基因的克隆及在花器官突变体中的表达分析

Cloning of PhalPI Gene from Phalaenopsis and Its Expression in Floral Organ Mutants

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