欢迎访问《植物研究》杂志官方网站,今天是 分享到:

植物研究 ›› 2020, Vol. 40 ›› Issue (6): 906-912.doi: 10.7525/j.issn.1673-5102.2020.06.013

• 研究报告 • 上一篇    

杨树SHMT基因家族分析及PtrSHMT9突变体创制

李冰, 程玉祥()   

  1. 东北林业大学林木遗传育种国家重点实验室,哈尔滨 150040
  • 收稿日期:2020-06-07 出版日期:2020-11-20 发布日期:2020-11-04
  • 通讯作者: 程玉祥 E-mail:chengyuxiang@nefu.edu.cn
  • 作者简介:李冰(1995—),女,硕士研究生,主要从事林木次生生长研究。
  • 基金资助:
    国家自然科学基金(31570580)

Analysis of SHMT Gene Family and Production of PtrSHMT9 Mutant in Populus trichocarpa

Bing LI, Yu-Xiang CHENG()   

  1. State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040
  • Received:2020-06-07 Online:2020-11-20 Published:2020-11-04
  • Contact: Yu-Xiang CHENG E-mail:chengyuxiang@nefu.edu.cn
  • About author:LI Bing(1995—),female,master,mainly engaged in tree secondary growth.
  • Supported by:
    National Natural Science Foundation of China(31570580)

摘要:

丝氨酸羟甲基转移酶(SHMT)在植物细胞一碳代谢途径上起着重要作用。我们识别毛果杨PtrSHMT家族有9个基因成员,eFP数据显示7个PtrSHMTs基因在多个组织内有转录表达,其中PtrSHMT9在木质部表达水平最高。进一步定量PCR和Aspwood检测显示,PtrSHMT9表达量在茎木质组织次生壁加厚阶段呈现高水平,这表明它可能参与杨树木材形成。用Cas9/gRNA基因编辑技术,制备PtrSHMT9被编辑产生的突变体,获得4个不同株系ptrshmt9敲除突变体。这些研究结果为深入探究树木SHMTPtrSHMT9功能,提供了基础信息和遗传材料。

关键词: 毛果杨, SHMT, ptrshmt9突变体, Cas9/gRNA

Abstract:

Serine hydroxy methyltransferases(SHMTs) played an important role in the one-carbon(C1) metabolism pathway in plant cells. We identified 9 SHMT genes from the genome of Populus trichocarpa. Populus eFP resource showed that 7 PtrSHMTs were expressed in multiple tissues, among which PtrSHMT9 was the most highly expressed gene in xylem. By analysis of RT-PCR and Aspwood, PtrSHMT9 had a high level in secondary wall thickening of stem wood tissue, suggested its involvement in poplar wood formation. Using Cas9/gRNA technique, PtrSHMT9-edited mutants were prepared and 4 different ptrshmt9 knockouts were obtained. Our results provided basic information and genetic material for exploring the function of SHMT and PtrSHMT9 in trees.

Key words: Populus trichocarpa, SHMT, ptrshmt9mutant, Cas9/gRNA

中图分类号: