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植物研究 ›› 2019, Vol. 39 ›› Issue (1): 87-95.doi: 10.7525/j.issn.1673-5102.2019.01.011

• 研究报告 • 上一篇    下一篇

基于SSR标记的不同优势等级云南松遗传多样性分析

蔡年辉1,2, 许玉兰1,2, 王亚楠2, 陈诗1, 汪梦婷2, 李根前1   

  1. 1. 西南林业大学西南山地森林资源保育与利用教育部重点实验室, 昆明 650224;
    2. 西南林业大学云南省高校林木遗传改良与繁育重点实验室, 昆明 650224
  • 收稿日期:2018-07-16 出版日期:2019-01-15 发布日期:2019-01-31
  • 通讯作者: 李根前 E-mail:lgenqian@public.km.cn
  • 作者简介:蔡年辉(1975-),男,博士研究生,副教授,主要从事森林培育的研究。
  • 基金资助:
    国家自然科学基金(31760204和31360189);云南省高校林木遗传改良与繁育重点实验室基金(YNGBT201702)

Genetic Diversity Characteristics in Different Dominance Hierarchies of Pinus yunnanensis Franch. Trees

CAI Nian-Hui1,2, XU Yu-Lan1,2, WANG Ya-Nan2, CHEN Shi1, WANG Meng-Ting2, LI Gen-Qian1   

  1. 1. Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Southwest Forestry University, Ministry of Education, Kunming 650224;
    2. Key Laboratory for Forest Genetic and Tree Improvement & Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming 650224
  • Received:2018-07-16 Online:2019-01-15 Published:2019-01-31
  • Supported by:
    National Natural Science Foundation of China(31760204 & 31360189);Key Laboratory For Forest Genetic and Tree Improvement & Propagation in Universities of Yunnan Province(YNGBT201702)

摘要: 基于SSR分子标记技术,按树高和胸径(地径)因子将样地内的植株分为优势木、中等木和劣势木3类,对不同生长优势等级云南松林木的遗传多样性变异特征进行了研究。结果表明:在林冠层,优势木的有效等位基因数、Shannon's信息指数、观测杂合度和期望杂合度分别为2.083、0.762、0.290和0.423,略高于中等木和劣势木;在更新层,有效等位基因数、Shannon's信息指数、观测杂合度和期望杂合度分别为2.063、0.774、0.272和0.410,除观测杂合度外,其余3个指标也均表现为优势木略高于中等木和劣势木。从差异显著性检验来看,不同生长优势等级云南松林木间的遗传多样性差异不显著,即林木生长分化对遗传多样性的影响不明显。

关键词: 优势等级, 遗传多样性, 云南松

Abstract: The trees were divided into the dominant trees, the intermediate trees and the suppressed trees according to the height and diameter(ground diameter) parameters. The variation of genetic diversity characteristics in different dominance hierarchies of Pinus yunnanensis Franch. population was studied by SSR molecular markers. In forest canopy layer, the mean number of effective alleles, Shannon's information index, observed heterozygosity and expected heterozygosity of dominant trees were 2.083, 0.762, 0.290 and 0.423, respectively, for the dominant trees, which slightly exceeded the intermediate and suppressed trees. In regeneration layer mean number of effective alleles, Shannon's information index, observed heterozygosity and expected heterozygosity were 2.063, 0.774, 0.272 and 0.410, respectively, for the dominant tree, which slightly exceeded the intermediate and suppressed trees except for observed heterozygosity. There were no significant differences among the different dominance hierarchy trees for the genetic diversity by the difference significance test of statistics method.

Key words: dominance hierarchy, genetic diversity, Pinus yunnanensis Franch

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