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植物研究 ›› 2018, Vol. 38 ›› Issue (4): 625-631.doi: 10.7525/j.issn.1673-5102.2018.04.019

• 研究简报 • 上一篇    下一篇

匀浆法提取沙枣果总黄酮工艺研究

张圆圆, 孟永斌, 徐蕾, 贾楠, 孟庆焕, 项凤影, 路祺   

  1. 东北林业大学森林植物生态学教育部重点实验室, 哈尔滨 150040
  • 收稿日期:2017-12-19 出版日期:2018-07-15 发布日期:2018-07-21
  • 通讯作者: 路祺 E-mail:luqi42700473@126.com
  • 作者简介:张圆圆(1991-),女,硕士研究生,主要从事生物有机活性分子研究。
  • 基金资助:
    中国高校基本科研业务费专项资金项目(2572016CA13);黑龙江省科学基金项目(C2018003)

Homogenate Extraction Process in Total Flavonoids from Elaeagnus angustifolia Fruit

ZHANG Yuan-Yuan, MENG Yong-Bin, XU Lei, JIA Nan, MENG Qing-Huan, XIANG Feng-Ying, LU Qi   

  1. Key Laboratory of Forest Plant Ecology of Northeast Forestry University, Ministry of Education, Harbin 150040
  • Received:2017-12-19 Online:2018-07-15 Published:2018-07-21
  • Supported by:
    Supported by the Fundamental Research Funds for the Central Universities(2572016CA13);Heilongjiang Science Fund Project(C2018003)

摘要: 沙枣常用于治疗脾胃虚弱、消化不良、肠炎腹泻、肺热咳嗽等疾病。为更好的开发利用沙枣植物资源,本研究通过传统水浴提取法和匀浆提取法的单因素对比实验,确定了匀浆法提取沙枣果总黄酮的提取方案。通过正交优化,获得匀浆法最优提取工艺为:乙醇浓度55%、料液比1∶30 g·mL-1、匀浆时间5 min。在优化工艺条件下,沙枣果总黄酮的得率为6.57%。本研究为沙枣果的综合开发利用提供理论依据。

关键词: 沙枣, 总黄酮, 匀浆提取

Abstract: Elaeagnus angustifolia is usually used in the treatment of spleen and stomach, indigestion, enteritis, diarrhea, cough and other diseases. In this study, the homogenate extraction method and the traditional method were compared by single factor experiment, and the method of homogenate extraction total flavonoids from E.angustifolia fruit was determined. The optimum technology conditions were as follows:ethanol concentration was 55%, solid-liquid ratio was 1:30 g·mL-1 and the extraction time was 5 min. Under the optimal conditions, the total flavonoids of E.angustifolia fruit yield rate was 6.57%. This study would provide a theoretical basis for the comprehensive development and utilization of E.angustifolia fruit.

Key words: Elaeagnus angustifolia, total flavonoids, homogenate extraction

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