高等植物二氢黄酮醇4-还原酶基因研究进展

doi:10.7525/j.issn.1673-5102.2018.04.020

摘要/Abstract

摘要： 花青素苷是影响植物花瓣呈色的重要色素，而花色是决定花卉观赏价值和商业价值的一个重要因素。在花青素苷的生物合成过程中，二氢黄酮醇4-还原酶（DFR）是花青素苷生物合成下游途径中的第一个关键的酶。因此，DFR在高等植物花色的形成过程中发挥极其重要的作用，是形成花青素苷的一个非常重要的调控点。DFR对3种二氢黄酮醇底物具有选择特异性，但决定DFR底物特异性的分子机制目前仍不十分清楚。该文简单概述了花青素苷生物合成途径及其转录调控机制，并结合作者的工作重点综述了DFR的底物特异性以及克隆的DFR基因在植物基因工程中的应用。

关键词: 花青素, 花青素苷, 类黄酮, 二氢黄酮醇4-还原酶, 基因工程

Abstract: Anthocyanins are important pigments for flower color of higher plants which is an important factor determining the appreciation value and economic value of floriculture plants. Dihydroflavonol 4-reductase(DFR) is a key enzyme involved in the late steps of the anthocyanin biosynthetic pathways. Thus, DFR is an important regulatory point for the formation of anthocyanins, which plays an important role in the formation of flower color. DFRs have different substrate specificities for three dihydroflavonols(dihydrokaempferol, dihydromyricetin and dihydroquercetin); however, the mechanism of substrate specificity determination in DFRs is still unclear. This paper summarizes the anthocyanin biosynthetic pathway and its transcriptional regulation mechanism. Combined with the authors' work, the substrate specificity of the dihydroflavonol 4-reductase(DFR) and the application of the cloned DFR gene in plant genetic engineering were extensively reviewed.

Key words: anthocyanidins, anthocyanins, flavonoids, dihydroflavonol 4-reductase(DFR), genetic engineering

中图分类号:

Q949.777.6

于婷婷, 倪秀珍, 高立宏, 韩国军, 朱长甫, 盛彦敏. 高等植物二氢黄酮醇4-还原酶基因研究进展[J]. 植物研究, 2018, 38(4): 632-640.

YU Ting-Ting, NI Xiu-Zhen, GAO Li-Hong, HAN Guo-Jun, ZHU Chang-Fu, SHENG Yan-Min. Advances in Study of Dihydroflavonol 4-Reductase(DFR) Genes of Higher Plants[J]. Bulletin of Botanical Research, 2018, 38(4): 632-640.

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