欢迎访问《植物研究》杂志官方网站,今天是 分享到:

植物研究 ›› 2013, Vol. 33 ›› Issue (2): 252-256.doi: 10.7525/j.issn.1673-5102.2013.02.023

• 论文 • 上一篇    

中药香薷不同部位总黄酮及3种主要黄酮成分含量分析

蒋华军1,2;林勇1,2;常秋3;王英姿1,2;黄志强4;刘仲华1*   

  1. 1.湖南农业大学国家植物功能成分利用工程技术研究中心,长沙 410128;2.湖南农业大学园艺园林学院,长沙 410128;3.江西农业大学食品科学与工程学院,南昌 330045;4.湖南省出入境检验检疫局,长沙 410004
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2013-03-20 发布日期:2013-03-20
  • 通讯作者: 刘仲华
  • 基金资助:
     

Analysis of the Contents of Total and Three Major Flavonoids in Different Tissues of Mosla chinensis

JIANG Hua-Jun;LIN Yong;CHANG Qiu;WANG Ying-Zi;HUANG Zhi-Qiang;LIU Zhong-Hua*   

  1. 1.National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients Hunan Agricultural University,Changsha 410128;2.College of Horticulture and Gardening of Hunan Agricultural University,Changsha 410128;3.Department of Food Science and Engineering of Jiangxi Agricultural University,Nanchang 330045;4.Hunan Entry-Exit Inspection and Quarantine Bureau,Changsha 410004
  • Received:1900-01-01 Revised:1900-01-01 Online:2013-03-20 Published:2013-03-20
  • Contact: LIU Zhong-Hua
  • Supported by:
     

摘要: 中药材中活性成分的含量是中药品质优劣的关键指标。本文建立了香薷中总黄酮和3种主要黄酮的分析方法,探索不同部位黄酮含量的差异,为香薷黄酮类成分的开发利用奠定基础。结果表明优化后的分光光度法对香薷总黄酮测定具有专一性、稳定性。高效液相色谱测定木犀草素、芹菜素和黄芩素-7-甲醚的方法在测定范围内表现出良好的线性关系(r>0.999);方法的回收率在98.77%~102.13%之间,RSD均小于5%;不同部位总黄酮的含量由高到低依次为:苞>尖>茎>根,且江西产香薷总黄酮的含量高于浙江产的香薷。这提示在工业生产上提取香薷黄酮类物质时尽量选择道地药材,同时可考虑弃去根部及茎的下端,以便提高浸提效率,增加经济效益。本试验建立的香薷中总黄酮和游离黄酮的测定方法准确、稳定,明确了香薷中总黄酮主要富集在花苞及附近,为进一步提高香薷资源的开发利用提供了理论依据和科学指导。

关键词: 香薷, 总黄酮, 木犀草素, 芹菜素, 黄芩素-7-甲醚, 反相高效液相色潽

Abstract: The content of active constituents in Chinese herbal medicine is a key indicator for judging their qualities. In this study, the methods were established for analyzing total and three major flavonoids in Mosla chinensis, respectively, and the differences of the flavonoid contents in different tissues were also compared, which will favour the exploitation of the flavonoids in M.chinensis. The results showed that the optimized spectrophotometry for the determination of total flavonoid content was specific and stable, and the HPLC method for the analysis of luteolin, apigenin and negletein presented a good linear relationship (r>0.999) in the range of the determination and the recovery ranges were 98.77%~102.13% with less than 5% RSDs. The study also found that the content of total flavonoids in different parts of M.chinensis from high to low was bud>tip>stem>root, and the total flavonoid content in M.chinensis from Jiangxi was higher than that from Zhejiang. These prompt that genunie medicinal materials should be selected for the industrial production of the flavonoids from M.chinensis as more as possible, and the root and the lower end of stem may be considered to discard, so as to improve the extraction efficiency and increase economic benefits. In brief, the developed methods are accurate and steady for detecting total and three major flavonoids in M.chinensis, and we also confirmed total flavonoids were mainly enriched in and near the bud of M.chinensis, which would provide a theoretical basis and scientific guidance for further exploiting the resources in M.chinensis.

Key words: Mosla chinensis, total flavonoids, luteolin, apigenin, negletein, RP-HPLC

中图分类号: