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植物研究 ›› 2016, Vol. 36 ›› Issue (3): 476-480.doi: 10.7525/j.issn.1673-5102.2016.03.023

• 研究报告 • 上一篇    

山里红总黄酮季节动态及其与气候因子相关性分析

罗猛1,2, 胡娇阳1,2, 宋卓悦1,2, 牟璠松1,2, 于雪莹1,2, 乔琪1,2, 阮鑫1,2, 杨璇1,2, 祖元刚1,2   

  1. 1. 东北林业大学森林植物生态学教育部重点实验室, 哈尔滨 150040;
    2. 林下经济资源研发与利用协同创新中心, 哈尔滨 150040
  • 收稿日期:2015-10-20 出版日期:2016-05-15 发布日期:2016-05-20
  • 通讯作者: 牟璠松,E-mail:fansong8867@nefu.edu.cn E-mail:fansong8867@nefu.edu.cn
  • 作者简介:罗猛(1979-),男,副研究员,主要从事植物活性成分研究。
  • 基金资助:
    中央高校基本科研业务费(2572015EA04,2572014CA08);黑龙江省博士后科研启动金(LBH-Q13003);国家自然科学基金(21403032)

Seasonal Variation of Total Flavonoids from Crataegus pinnatifida and Correlation Analysis of Climatic Factors

LUO Meng1,2, HU Jiao-Yang1,2, SONG Zhuo-Yue1,2, MU Fan-Song1,2, YU Xue-Ying1,2, QIAO Qi1,2, RUAN Xin1,2, YANG Xuan1,2, ZU Yuan-Gang1,2   

  1. 1. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040;
    2. The Development and Utilization Collaborative Innovation Center of Forest Economic Resources, Harbin 150040
  • Received:2015-10-20 Online:2016-05-15 Published:2016-05-20

摘要: 研究山里红中总黄酮含量随季节动态变化的规律,明确影响其总黄酮含量的气候因子。采收2013年5~10月的山里红,采用超声辅助提取法从山里红中提取总黄酮,三氯化铝比色法检测总黄酮的含量,并对山里红叶总黄酮含量与气候因子进行相关性分析。山里红不同部位总黄酮含量差异较大,9月份山里红叶黄酮类成分的含量最高,其值为31.52mg·g-1。统计九月份山里红叶总黄酮含量与气候因子之间相关性分析,结果表明总黄酮含量与温度相关性显著。综合考虑,选择9月份为山里红叶的最佳采收期。该结果可为山里红叶总黄酮的深入开发利用奠定理论基础。

关键词: 山里红, 总黄酮, 气候因子, 季节动态, 相关性

Abstract: We determined the contents of total flavonoids from Crataegus pinnatifida(C.pinnatifida) according to seasonal dynamics to find out the climate dominant climatic factors which influence the total flavonoids content(TFC) of C.pinnatifida. We studied the C.pinnatifida from May to October 2013, and used ultrasound-assisted extraction(UAE) to extract TFC from C.pinnatifida, and determined TFC by aluminum chloride colorimetric method. We analyzed the correlation between the TFC and climatic factors. TFC in different parts of C.pinnatifida had significant difference. TFC from C.pinnatifida leaves increase continuously and reach the highest point in September, with the value of 31.52 mg·g-1. A positive correlation was between the content of total flavonoid and temperature. September was the best harvest time for C.pinnatifida leaves. Our result provides a theoretical basis for development and utilization of total flavonoids in C.pinnatifida.

Key words: Crataegus pinnatifida, total flavonoids, climatic factors, seasonal variation, correlation

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