白桦BpBEE1基因启动子的表达特性分析

doi:10.7525/j.issn.1673-5102.2018.02.012

植物研究 ›› 2018, Vol. 38 ›› Issue (2): 247-253.doi: 10.7525/j.issn.1673-5102.2018.02.012

白桦BpBEE1基因启动子的表达特性分析

许思佳¹, 颜斌¹, 董京祥², 武丹阳¹, 李慧玉¹

1. 东北林业大学林木遗产育种国家重点实验室, 哈尔滨 150040;
2. 东北林业大学, 哈尔滨 150040

收稿日期:2017-07-18 出版日期:2018-03-15 发布日期:2018-03-23
通讯作者: 李慧玉 E-mail:lihuiyu0519@aliyun.com
作者简介:许思佳(1992-),女,硕士研究生,主要从事林木遗传育种研究。
基金资助:
国家重点研发计划（2017YFD0600603）

Analysis of BpBEE1 Gene Promoter in Betula platyphylla

XU Si-Jia¹, YAN Bin¹, DONG Jing-Xiang², WU Dan-Yang¹, LI Hui-Yu¹

1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040;
2. Northeast Forest University, Harbin 150040

Received:2017-07-18 Online:2018-03-15 Published:2018-03-23
Supported by:
National Key R&D Plan(2017YFD0600603)

摘要/Abstract

摘要： Brassinolide Enhanced Expression1（BEE1）基因属于bHLH转录因子家族，是调控油菜素内酯信号转导的重要元件。本研究克隆了BpBEE1基因的启动子，通过生物信息学对启动子顺式作用元件进行分析发现：BEE1启动子序列中含有与多种激素应答相关的元件；对转Promoter-BEE1基因拟南芥株系进行组织特异性染色和激素处理，结果表明：转基因株系中GUS染色在叶脉和根系中染色较深，MeJA、SA、BL和ABA处理后GUS活性增强，尤其是MeJA、SA、BL处理后的GUS染色最深。以上结果说明白桦BpBEE1基因参与激素应答等生物学过程，并对植物的生长发育有一定的调控作用。

关键词: 白桦, BpBEE启动子, 表达特性, 激素

Abstract: Brassinolide Enhanced Expression(BEE) genes belong to bHLH transcription factors family, and were involved in Brassinolide signal pathway. In this study, the promoter of BpBEE1 gene was cloned, and regulatory elements in the promoter regions were analyzed using the online program PLACE. The results showed that the BEE1 promoter sequence contained various hormone responses elements. The results showed that the GUS activity was increased in the veins and roots, and the activity of promoter was enhanced after MeJA, SA, BL and ABA treatment, especially MeJA, SA and BL treatment. We propose that BpBEE1 is involved in hormone response, and play a role in the process of plant growth and development.

Key words: Betula platyphylla, BpBEE promoter, Genetic transformation, honmones

中图分类号:

S792

许思佳, 颜斌, 董京祥, 武丹阳, 李慧玉. 白桦BpBEE1基因启动子的表达特性分析[J]. 植物研究, 2018, 38(2): 247-253.

XU Si-Jia, YAN Bin, DONG Jing-Xiang, WU Dan-Yang, LI Hui-Yu. Analysis of BpBEE1 Gene Promoter in Betula platyphylla[J]. Bulletin of Botanical Research, 2018, 38(2): 247-253.

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白桦BpBEE1基因启动子的表达特性分析

Analysis of BpBEE1 Gene Promoter in Betula platyphylla

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