植物研究 ›› 2024, Vol. 44 ›› Issue (4): 576-589.doi: 10.7525/j.issn.1673-5102.2024.04.010
• 生理与生态 • 上一篇
任艳君1, 郭晓瑞1, 于子煊1, 吴可心1, 孙宇2, 陈宁1, 油乔木1, 邢凯鑫3()
收稿日期:
2023-12-22
出版日期:
2024-07-20
发布日期:
2024-07-09
通讯作者:
邢凯鑫
E-mail:125291077@qq.com
作者简介:
任艳君(1995—),女,硕士研究生,主要从事植物资源研究。
基金资助:
Yanjun REN1, Xiaorui GUO1, Zixuan YU1, Kexin WU1, Yu SUN2, Ning CHEN1, Qiaomu YOU1, Kaixin XING3()
Received:
2023-12-22
Online:
2024-07-20
Published:
2024-07-09
Contact:
Kaixin XING
E-mail:125291077@qq.com
摘要:
为了探究刺五加(Acanthopanax senticosus)种子的休眠类型以及种子萌发过程中生理和代谢特征,以东北地区刺五加种子为研究对象,分别在不同温度条件(20 ℃,4 ℃,20 ℃/4 ℃)下对种子进行140 d沙藏层积处理。通过观察胚的表型变化,筛选出5个典型时期。研究5个典型时期种子中的内源激素、主要营养物质及其水解酶、抗氧化系统和代谢组学的动态变化规律。结果表明:(1)刺五加种子吸水性能良好,20 ℃恒温条件只能促进胚的伸长,不能萌发。4 ℃恒温条件下胚率无增长。只有0~110 d保持20 ℃恒温,110~140 d保持4 ℃恒温,胚率增长且种子能够萌发。(2)随着胚率增长,赤霉素含量上升,脱落酸含量下降,吲哚乙酸、吲哚丁酸和萘乙酸含量均下降;脂肪含量下降,可溶性糖含量上升。脂肪含量与脂肪水解酶活性呈显著负相关,与可溶性糖含量呈极显著负相关。(3)共筛选出92个差异代谢物,碳代谢、能量代谢和抗氧化代谢是主要的差异代谢通路。此外,倍半萜和三萜生物合成、脂肪酸生物合成、甘油脂类代谢等也存在差异。总之,刺五加种子属于形态生理性休眠,变温层积是打破种子休眠的必要条件,内源激素能够调控种子的休眠与萌发。脂肪等大分子营养物质被水解酶分解,为种子萌发提供物质和能量,这也是种子萌发的必要条件之一。该研究可为鉴定刺五加种子休眠类型以及人工繁殖提供理论依据。
中图分类号:
任艳君, 郭晓瑞, 于子煊, 吴可心, 孙宇, 陈宁, 油乔木, 邢凯鑫. 变温层积不同阶段刺五加种子萌发生理及代谢特点[J]. 植物研究, 2024, 44(4): 576-589.
Yanjun REN, Xiaorui GUO, Zixuan YU, Kexin WU, Yu SUN, Ning CHEN, Qiaomu YOU, Kaixin XING. Characteristics of Temperature Stratification on Germination Physiology and Metabolism of Acanthopanax senticosus Seeds at Different Stages[J]. Bulletin of Botanical Research, 2024, 44(4): 576-589.
表1
生理指标的相关性
生理指标 Physiological indexes | 胚率 Embryo rate | 粗脂肪 Crude fat | 可溶性糖 Soluble sugar | 脂肪水解酶 Lipase | 可溶性蛋白 Soluble protein | 蛋白质水解酶 Proteolytic enzyme | 超氧阴离子 产生速率 ROS generation rate | 超氧化物 歧化酶 SOD | 过氧化氢酶 CAT | 过氧化物酶 POD |
---|---|---|---|---|---|---|---|---|---|---|
胚率 Embryo rate | 1 | -0.801** | 0.970** | 0.437 | -0.039 | -0.702** | -0.478 | 0.747** | 0.331 | 0.644** |
粗脂肪 Crude fat | 1 | -0.797** | -0.607* | -0.308 | 0.307 | 0.469 | -0.698** | -0.341 | -0.348 | |
可溶性糖 Soluble sugar | 1 | 0.600* | 0.114 | -0.565* | -0.338 | 0.692** | 0.181 | 0.727** | ||
脂肪水解酶 Lipase | 1 | 0.807** | 0.111 | 0.332 | 0.106 | -0.051 | 0.595* | |||
可溶性蛋白 Soluble protein | 1 | 0.383 | 0.603* | -0.346 | 0.05 | 0.274 | ||||
蛋白质水解酶 Proteolytic enzyme | 1 | 0.336 | -0.353 | -0.655** | -0.545* | |||||
超氧阴离子产生速率 ROS generation rate | 1 | -0.832** | -0.149 | 0.253 | ||||||
超氧化物歧化酶 SOD | 1 | -0.033 | 0.103 | |||||||
过氧化氢酶 CAT | 1 | 0.168 | ||||||||
过氧化物酶 POD | 1 |
1 | NONOGAKI H.Seed dormancy and germination-emerging mechanisms and new hypotheses[J].Frontiers in Plant Science,2014,5:233. |
2 | YAO M Q, CHEN W W, KONG J H,et al. METHYLTRANSFERASE1 and ripening modulate vivipary during tomato fruit development[J].Plant Physiology,2020,183(4):1883-1897. |
3 | BASKIN J M, BASKIN C C.A classification system for seed dormancy[J].Seed Science Research,2004,14(1):1-16. |
4 | 吉浴芳,宋松泉,刘军,等.毛叶山桐子种子休眠与萌发特性的研究[J].中国野生植物资源,2023,42(6):23-28. |
JI Y F, SONG S Q, LIU J,et al.Study on characteristics of dormancy and germination in Idesia polycarpa seeds[J].Chinese Wild Plant Resources,2023,42(6):23-28. | |
5 | 李涵,阎君,吴珏,等.低温诱导芹菜种子次生休眠及其调控机制[J].南京农业大学学报,2023,46(5):833-840. |
LI H, YAN J, WU Y,et al.Low temperature induced secondary dormancy of celery seeds and its regulatory mechanism[J].Journal of Nanjing Agricultural University,2023,46(5):833-840. | |
6 | 李金航,葛丽丽,杜凤国,等.天女木兰研究热点及展望:基于Citespace的可视化计量[J].北华大学学报(自然科学版),2023,24(6):712-721. |
LI J H, GE L L, DU F G,et al.Research hotspots and prospects of Oyama sieboldii:based on visuabibliometrics via Citespace[J].Journal of Beihua University(Natural Science Edition),2023,24(6):712-721. | |
7 | 徐亮,李建东,殷萍萍,等.野生大豆种皮形态结构和萌发特性的研究[J].大豆科学,2009,28(4):641-646. |
XU L, LI J D, YIN P P,et al.Testa morphology structure and germination characteristic of Glycine soja [J].Soybean Science,2009,28(4):641-646. | |
8 | 邢露,李铁华,何功秀,等.紫楠种子的休眠与萌发特性[J].中南林业科技大学学报,2023,43(6):43-50. |
XING L, LI T H, HE G X,et al.Seed dormancy and germination characteristics of Phoebe sheareri [J].Journal of Central South University of Forestry and Technology,2023,43(6):43-50. | |
9 | 王炎,赵敏,俞加林.北五味子种子休眠特性及内源抑制物质的研究[J].中国中药杂志,1997(1):11-13. |
WANG Y, ZHAO M, YU J L.A study on the dormancy characteristic and inner inhibitory substances of Fructus schisandrae [J].China Journal of Chinese Materia Medica,1997(1):11-13. | |
10 | 赵阳,刘迪,樊磊,等.东北刺人参种子实用催芽技术研究[J].现代园艺,2019(2):15-17. |
ZHAO Y, LIU D, FAN L,et al.The analysis of germination effectiveness of Oplopanax elatus seed with different stratification treatment[J].Modern Horticulture,2019(2):15-17. | |
11 | 缪璐琳,谢艳,刘森,等.黄精的繁殖及种植技术研究进展[J].种子科技,2022,40(2):70-72. |
MIAO L L, XIE Y, LIU S,et al.Research progress on propagation and planting techniques of Polygonatum sibiricum [J].Seed Science and Technology,2022,40(2):70-72. | |
12 | JIN L, SCHMIECH M, GAAFARY M E,et al.A comparative study on root and bark extracts of Eleutherococcus senticosus and their effects on human macrophages[J].Phytomedicine,2020,68:153181. |
13 | SONG C, LI S J, DUAN F Y,et al.The therapeutic effect of Acanthopanax senticosus components on radiation-induced brain injury based on the pharmacokinetics and neurotransmitters[J].Molecules,2022,27(3):1106. |
14 | 韩春霞,李廷利,郭冷秋.刺五加水煎剂改善睡眠作用研究[J].中华中医药学刊,2007,25(10):2084-2085. |
HAN C X, LI T L, GUO L Q.Sleep effects of aqueous extract manyprickle Acanthopanax senticosus root[J].Chinese Archives of Traditional Chinese Medicine,2007,25(10):2084-2085. | |
15 | 卞宏生,蔡江莹,杨凡静暄.刺五加总苷及刺五加苷B对持续黑暗条件下果蝇睡眠-觉醒节律的量效关系研究[J].中国药物依赖性杂志,2020,29(3):231-236. |
BIAN H S, CAI J Y, YANG F J X.Study on the dose-effect relationship of acanthopanax total glycoside and eleutheroside B on sleep-wake rhythm in Drosophila melanogaster under continuous darkness[J].Chinese Journal of Drug Dependence,2020,29(3):231-236. | |
16 | 周德本.刺五加种子的处理方法[J].林业科技通讯,1979(6):2. |
ZHOU D B.Treatment of Acanthopanax senticosus seeds[J].Forest Science and Technology,1979(6):2. | |
17 | 周德本,王桂堂,王云霞.刺五加的种子与扦插繁殖[J].中草药,1981,12(8):34-36. |
ZHOU D B, WANG G T, WANG Y X.Seeds and cutting propagation of Acanthopanax Senticosus [J].Chinese Traditional and Herbal Drugs,1981,12(8):34-36. | |
18 | 刘俊义,薛茂贤,程丽雅,等.刺五加种子育苗技术的研究[J].中国林副特产,1992(2):5-6. |
LIU J Y, XUE M X, CHENG L Y,et al.Study on seed seedling raising technology of Acanthopanax senticosus [J].Forest By-Product and Speciality in China,1992(2):5-6. | |
19 | 韩承伟,明兆清,闫思维,等.刺五加种子催芽处理方法的研究[J].中国林副特产,1994(1):1-2. |
HAN C W, MING Z Q, YAN S W,et al.Study on the method of accelerating the germination of Acanthopanax senticosus seeds[J].Forest By-Product and Speciality in China,1994(1):1-2. | |
20 | 许怡玲.刺五加种子后熟期间的发育过程与代谢的相关[J].中国林副特产,1989(4):7-10. |
XU Y L.Correlation between the development process and metabolism of Acanthopanax senticosus seeds during post-ripening[J].Forest By-Product and Speciality in China,1989(4):7-10 | |
21 | 田国伟,刘林德,王仲礼,等.刺五加种子结构、后熟作用及其细胞化学研究[J].西北植物学报,1999,19(1):7-13. |
TIAN G W, LIU L D, WANG Z L,et al.Studies on seed structure,after-ripening and cytochemistry of Acanthopanax senticosus [J].Acta Botanica Boreali-Occidentalia Sinica,1999,19(1):7-13. | |
22 | 赵敏,王炎,康莉.刺五加果实及种子内源萌发抑制物质活性的研究[J].中国中药杂志,2001,26(8):534-538. |
ZHAO M, WANG Y, KANG L.Studies on the activity of endogenous germination inhibitors in Acanthopanax senticosus fruits and seeds[J].China Journal of Chinese Materia Medica,2001,26(8):534-538. | |
23 | 李玉德.药剂处理对刺五加种子发芽率的影响[J].农业科技与装备,2014(1):18-19. |
LI Y D.Effect of chemical treatment on the germination rate of Acanthopanax senticosus seeds[J].Agricultural Science and Technology and Equipment,2014(1):18-19. | |
24 | 高成华.外源GA对刺五加种子后熟过程中内源激素含量变化的影响[J].种子,2016,35(1):35-37. |
GAO C H.Effect of exogenous GA on the changes of endogenous hormone content in Acanthopanax senticosus seeds during post-ripening[J].Seed,2016,35(1):35-37. | |
25 | 韩笑,李悦,逄东霖,等.化学方法处理对刺五加种子萌发特性的影响[J].吉林农业,2019(18):46-48. |
HAN X, LI Y, PANG D L,et al.Effects of chemical treatments on the germination characteristics of Acanthopanax senticosus seeds[J].Jilin Agriculture,2019(18):46-48. | |
26 | 沈宏伟,张爽,付士朋,等.内生真菌对刺五加种子萌发过程激素及酶含量变化的影响[J].中草药,2019,50(3):716-721. |
SHEN H W, ZHANG S, FU S P,et al.Effects of endophytic fungi on the changes of hormone and enzyme contents during the germination of Acanthopanax senticosus seeds[J].Chinese Traditional and Herbal Drugs,2019,50(3):716-721. | |
27 | 沈宏伟,张爽,李佳宾,等.不同浓度的外源NO对刺五加种子激素及酶含量变化的影响[J].中国实验方剂学杂志,2019,25(3):137-142. |
SHEN H W, ZHANG S, LI J B,et al.Effects of exogenous NO with different concentrations on the changes of hormones and enzymes in Acanthopanax senticosus seeds[J].Chinese Journal of Experimental Traditional Medical Formulae,2019,25(3):137-142. | |
28 | 付士朋,沈宏伟,王谦博,等.基于灰色关联度法分析刺五加种子中胚率、内源激素和酶活力的相关性[J].中国实验方剂学杂志,2020,26(4):145-151. |
FU S P, SHEN H W, WANG Q B,et al.Correlation analysis of embryo rate,endogenous hormones and enzyme activity in Acanthopanax senticosus seeds based on grey correlation degree method[J].Chinese Journal of Experimental Traditional Medical Formulae,2020,26(4):145-151. | |
29 | DZONDO-GADET M, NZIKOU J M, KIMBONGUILA A,et al.Solvent and enzymatic extraction of Safou and Kolo oils[J].European Journal of Lipid Science and Technology,2004,106(5):289-293. |
30 | FENG J, DONG P, LI R M,et al.Effects of wood fiber properties on mold resistance of wood polypropylene composites[J].International Biodeterioration & Biodegradation,2019,140:152-159. |
31 | WU Y, SHEN Y B.Dormancy in Tilia miqueliana is attributable to permeability barriers and mechanical constraints in the endosperm and seed coat[J].Brazilian Journal of Botany,2021,44(3):725-740. |
32 | PAN C L, LU H L, YU J F,et al.Identification of cadmium-responsive Kandelia obovata SOD family genes and response to Cd toxicity[J].Environmental and Experimental Botany,2019,162:230-238. |
33 | LI P X, HU H L, LUO S F,et al.Shelf life extension of fresh lotus pods and seeds(Nelumbo nucifera Gaertn.) in response to treatments with 1-MCP and lacquer wax[J].Postharvest Biology and Technology,2017,125:140-149. |
34 | 彭旭阳,董成虎,赵晓梅,等.臭氧处理对冬枣贮藏品质的影响[J].食品研究与开发,2023,44(21):26-32. |
PENG X Y, DONG C H, ZHAO X M,et al.Effect of ozone treatment on storage quality of winter jujube[J].Food Research and Development,2023,44(21):26-32. | |
35 | 杜丹丹.平茬对杜仲幼苗及腋芽生长调节的机理研究[D].哈尔滨:东北林业大学,2021. |
DU D D.Study on the growth regulation mechanism of pruned on the Eucommia ulmoides Oliv.saplings and axillary buds[D].Harbin:Northeast Forestry University,2021. | |
36 | 刘洋.应用代谢组学对比研究两种黄芪响应UV-B和干旱胁迫的代谢基础[D].哈尔滨:东北林业大学,2018. |
LIU Y.Comparative study on the metabolic basis of two Astragalus membranaceus species in response to UV-B and drought stress by metabonomics[D].Harbin:Northeast Forestry University,2018. | |
37 | CHEN Y, XIANG Z P, LIU M,et al.ABA biosynthesis gene OsNCED3 contributes to preharvest sprouting resistance and grain development in rice[J].Plant,Cell and Environment,2023,46(4):1384-1401. |
38 | CHEN K, YIN Y T, DING Y R,et al.Characterization of oil body and starch sranule dynamics in developing seeds of Brassica napus [J].International Journal of Molecular Sciences,2023,24(4):4201. |
39 | JURDAK R,DE ALMEIDA GARCIA RODRIGUES G, CHAUMONT N,et al.Intracellular reactive oxygen species trafficking participates in seed dormancy alleviation in Arabidopsis seeds[J].New Phytologist,2022,234(3):850-866. |
40 | 徐绍娣,李辉,张顺捷.刺五加实生苗生产中存在问题与分析[J].中国林副特产,2017(5):95-96. |
XU S D, LI H, ZHANG S J.Problems and analysis in the production of Acanthopanax senticosus seedlings[J].Forest By-Product and Speciality in China,2017(5):95-96. | |
41 | 崔立勇,佟庆,史伟国,等.光照对刺五加种子萌发和幼苗生长的影响[J].西北农业学报,2015,24(8):157-164. |
CUI L Y, TONG Q, SHI W G,et al.Effects of light intensity on seed germination and seedling growth of Acanthopanax senticosus [J].Acta Agriculturae Boreali-occidentalis Sinica,2015,24(8):157-164. | |
42 | 邢朝斌,沈海龙,黄剑,等.不同温度、时间层积处理对刺五加种子发芽的影响[J].辽宁林业科技,2006(5):9-11. |
XING C B, SHEN H L, HUANG J,et al.Effects of stratification at different temperature and time on seed germination of Eleutherococcus senticosus [J].Liaoning Forestry Science and Technology,2006(5):9-11. | |
43 | 马淑兰.刺五加种子休眠机理研究[D].长春:吉林农业大学,2006. |
MA S L.Study on the dormancy mechanism of Acanthopanax senticosus seed[D].Changchun:Jilin Agricultural University,2006. | |
44 | 李晓琳,黄璐琦,李颖,等.刺五加种子质量分级标准研究[J].现代中药研究与实践,2017,31(2):50-53. |
LI X L, HUANG L Q, LI Y,et al.Research on seed quality grading standard of Acanthopanax senticosus seeds[J].Research and Practice on Chinese Medicines,2017,31(2):50-53. | |
45 | 潘学军,李德燕,张文娥.贵州野生刺葡萄和腺枝葡萄种子萌发生理特性的研究[J].北方园艺,2010(6):15-17. |
PAN X J, LI D Y, ZHANG W E.The germinating physiological characteristic of wild vitis(V.davidii and V.adenoclada) native in Guizhou Province[J].Northern Horticulture,2010(6):15-17. | |
46 | ZHAO S, GARCIA D, ZHAO Y L,et al.Hydro-electro hybrid priming promotes carrot(Daucus carota L.) seed germination by activating lipid utilization and respiratory metabolism[J].International Journal of Molecular Sciences,2021,22(20):11090. |
47 | 张彩华.胡杨和灰杨种子萌发盐胁迫的转录组研究[D].兰州:兰州大学,2022. |
ZHANG C H.Transcriptomic analyses of seed germination under salt stress in Populus euphratica and Populus pruinosa [D].Lanzhou:Lanzhou University,2022. | |
48 | 茹刚,陈学林,王桠楠,等.西伯利亚乌头种子休眠及破除方法的研究[J].中药材,2020,43(2):280-285. |
RU G, CHEN X L, WANG Y N,et al.Study on seed dormancy and breaking methods of Aconitum sibiricum [J].Journal of Chinese Medicinal Materials,2020,43(2):280-285. | |
49 | WANG J J, SUN N, ZHANG F F,et al.SAUR17 and SAUR50 differentially regulate PP2C-D1 during apical hook development and cotyledon opening in Arabidopsis [J].The Plant Cell,2020,32(12):3792-3811. |
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