黄独微型块茎低温离体保存的GC/MS代谢组学分析

doi:10.7525/j.issn.1673-5102.2018.02.011

摘要/Abstract

摘要： GC/MS检测方法采用初步探明黄独低温离体保存微型块茎的差异代谢物。与黄独微型块茎25℃离体保存相比较，黄独微型块茎4℃离体保存的差异性代谢物有丙氨酸（Alanine）、儿茶素（Catechin）、N，N-双（2-羟乙基）甲胺（N，N-Di-（2-Hydroxyethyl）-methanamine）、水杨酸（Salicylic acid）、柠檬酸（Citric acid）和山梨糖（Sorbose）等。在黄独微型块茎4℃离体保存中，丙氨酸（Alanine）参与氰基氨基酸代谢；儿茶素（Catechin）参与次生代谢产物生物合成、黄酮类化合物的生物合成和苯丙素的生物合成；水杨酸（Salicylic acid）参与多环芳烃降解、微生物在不同环境中的代谢、植物激素信号转导、次生代谢产物生物合成、二恶英降解、苯丙氨酸代谢、芳烃降解、植物激素生物合成、铁载体组非核糖体肽合成和苯丙素的生物合成等。柠檬酸（Citric acid）参与来自鸟氨酸、赖氨酸和烟酸的生物碱生物合成、组氨酸和嘌呤的生物碱生物合成、微生物在不同环境中的代谢、植物次生代谢产物的生物合成、2-氧代羧酸代谢、萜类和类固醇的生物合成、原核生物固碳途径、次生代谢产物生物合成、来自莽草酸途径的生物碱生物合成、来自萜类化合物和聚酮的生物碱生物合成、柠檬酸循环（TCA循环）、植物激素生物合成、乙醛酸和二羧酸代谢、双组分系统、苯丙素的生物合成以及来自鸟氨酸，赖氨酸和烟酸的生物碱生物合成等。黄独低温离体保存微型块茎差异代谢物的初步发现为进一步了解其低温离体保存的分子机制奠定了基础，也为低温离体保存黄独微型块茎的破除休眠以及其后续萌发提供了理论依据。

关键词: 黄独, 微型块茎, 低温离体保存, GC/MS, 代谢组学分析

Abstract: We applied GC/MS detection method to different metabolites of Dioscorea bulbifera L. microtubers conserved in vitro at low temperature. Compared with D.bulbifera L. microtubers conserved in vitro at 25℃, different metabolites of D.bulbifera L. microtubers conserved in vitro at 4℃ had alanine, catechin, N,N-Di-(2-Hydroxyethyl)-methanamine, salicylic acid, citric acid and sorbose. In the conservation in vitro of microtuber at 4℃, alanine mainly involved in cyanoamino acid metabolism; Catechin mainly involved in biosynthesis of secondary metabolites, flavonoid biosynthesis and biosynthesis of phenylpropanoids; Salicylic acid mainly involved in polycyclic aromatic hydrocarbon degradation, microbial metabolism in diverse environments, plant hormone signal transduction, biosynthesis of secondary metabolites, dioxin degradation, phenylalanine metabolism, degradation of aromatic compounds, biosynthesis of plant hormones, biosynthesis of siderophore group non-ribosomal peptides and biosynthesis of phenylpropanoids; Citric acid mainly involved in biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid, biosynthesis of alkaloids derived from histidine and purine, microbial metabolism in diverse environments, biosynthesis of plant secondary metabolites, 2-Oxocarboxylic acid metabolism, biosynthesis of terpenoids and steroids, carbon fixation pathways in prokaryotes, biosynthesis of secondary metabolites, biosynthesis of alkaloids derived from shikimate pathway, biosynthesis of alkaloids derived from terpenoid and polyketide, citrate cycle(TCA cycle), biosynthesis of plant hormones, glyoxylate and dicarboxylate metabolism, two-component system, biosynthesis of phenylpropanoids and biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid. The preliminary findings of different metabolites of D.bulbifera L. microtubers conserved in vitro at low temperature would lay the foundation for further understanding the molecular mechanism of conservation in vitro at low temperature and provide a theoretical basis for low temperature conservation breaking dormancy and its subsequent germination D.bulbifera L. microtubers.

Key words: Dioscorea bulbifera L., microtuber, low temperature conservation, GC/MS, metabonomics analysis

中图分类号:

R932

尹明华, 邓红根, 蒋妍, 万琳, 吴丽霞, 凌飞, 汪金华. 黄独微型块茎低温离体保存的GC/MS代谢组学分析[J]. 植物研究, 2018, 38(2): 238-246.

YIN Ming-Hua, DENG Hong-Gen, JIANG Yan, WAN Lin, WU Li-Xia, LING Fei, WANG Jin-Hua. GC/MS Metabonomics Analysis of Dioscorea bulbifera L. Microtubers Conserved in vitro at Low Temperature[J]. Bulletin of Botanical Research, 2018, 38(2): 238-246.

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