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植物研究 ›› 2001, Vol. 21 ›› Issue (3): 375-379.

• 论文 • 上一篇    下一篇

刺五加花粉活力和柱头可授性的研究

刘林德1,3, 张洪军2, 祝宁2, 申家恒1   

  1. 1. 烟台师范学院生物科学与技术系, 烟台 264025;
    2. 东北林业大学森林资源与环境学院, 哈尔滨 150040;
    3. 南开大学生命科学学院, 天津 300071
  • 收稿日期:2001-02-16 出版日期:2001-09-15 发布日期:2016-06-14
  • 作者简介:刘林德(1964-), 男, 教授, 博士后, 主要从事植物生态学、植物生殖生物学和遗传学教学与研究。
  • 基金资助:
    黑龙江省自然科学基金资助项目。同时得到国家自然科学基金(39391500 和39470130)和林业部重点课题(96-28)资助。

POLLEN VIABILITY AND STIGMA RECEPTIVITYOF ELEUTHEROCOCCUS SENTICOSUS (ARALIACEAE)

LIU Lin-de1,3, ZHANG Hong-jun2, ZHU Ning2, SHEN Jia-heng1   

  1. 1. Department of Bioscience and Biotechnology, Yantai Normal University, Yantai 264025;
    2. College of Forest Resources and Environment, Northeast Forestry University, Harbin 150040;
    3. College of Life Sciences, Nankai University, Tianjin 300071
  • Received:2001-02-16 Online:2001-09-15 Published:2016-06-14

摘要: 用TTC 法测定了刺五加花粉的活力和寿命, 用联苯胺-过氧化氢法、开花后套袋法测定了其柱头可授期, 用扫描电子显微镜观察了刺五加柱头的亚显微形态特征。结果表明, 刺五加长花丝植株的花粉活力比中花丝植株的高;花粉活力及其下降快慢随生境不同而有所差异;通常情况下, 花粉寿命约为4 天。刺五加长花丝植株的柱头一直不具有可授性;中花丝植株和短花丝植株的柱头, 因所处生境不同, 在开花后5~7、6~8 或7~9 天具可授性;生境越郁闭, 柱头具可授性的时间越晚。具可授性的柱头有独特的形态特征, 即:柱头外翻、5 或4 裂、湿性、乳突细胞呈猫耳朵状。

关键词: 刺五加, 花粉, 花粉活力, 柱头, 柱头可授性, 扫描电子显微镜

Abstract: Pollen viability and life-span of Eleutherococcus senticosus was evaluated by TTC (2, 3, 5-triphenyl tetrazolium chloride)test, its stigma receptivity was estimated by benzidine-H 2O 2 method and bagging, and the stigma submicroscopic morphology was examined with scanning electron microscopy (SEM). The results as follows:In same habtat, the pollen viability of long-filament (male)E. senticosus was higher than that of middle-filament (bisexual)E. senticosus, the life span of both of them was about 4 days. Moreover, the pollen viability and its decline rate were different according to different plants and different habitats. The stigma of long-filament E. senticosus hadn't receptivity forever, the short-filament (female)and middle-filament (bisexual)E. senticosus had stigma receptivity at 5~7, 6~8, or 7~9 days after blooming according to different habitats, and the more the canopy closure was, the later the stigma receptivity began. When the stigmas began to receive pollens, they were wet, dehiscent and 5 or 4-lobed, and the stigma papillae were kitten's ear-like shape.

Key words: Eleutherococcus senticosus, pollen, pollen viability, stigma, stigma receptivity, scanning electron microscopy