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植物研究 ›› 2022, Vol. 42 ›› Issue (2): 268-277.doi: 10.7525/j.issn.1673-5102.2022.02.012

• 生理与生态 • 上一篇    下一篇

刺五加和短梗五加叶片初生代谢的发育特异性调控研究

宋莹1, 吴可心2, 邵文达1, 刘昱利1, 刘佳3, 刘洋2, 唐中华1()   

  1. 1.东北林业大学化学化工与资源利用学院,哈尔滨 150040
    2.东北林业大学林学院,哈尔滨 150040
    3.中国科学院东北地理与农业生态研究所,哈尔滨 150040
  • 收稿日期:2021-05-18 出版日期:2022-03-20 发布日期:2022-02-22
  • 通讯作者: 唐中华 E-mail:tangzh@nefu.edu.cn
  • 作者简介:宋莹(1995—),女,硕士研究生,主要从事药用植物生理生态研究工作。
  • 基金资助:
    科技部基础性工作专项(2019FY100505);国家林业局科学技术推广项目(201912);生物安全与遗传资源管理项目(KJZXZH202001)

Development-specific Investigation of Leaf Primary Metabolism of Acanthopanax senticosus and Acanthopanax sessiliflorus

Ying Song1, Kexin Wu2, Wenda Shao1, Yuli Liu1, Jia Liu3, Yang Liu2, Zhonghua Tang1()   

  1. 1.College of Chemistry Chemical Engineering and Resource Utilization,Northeast Forestry University,Harbin 150040
    2.School of Forestry,Northeast Forestry University,Harbin 150040
    3.Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Harbin 150040
  • Received:2021-05-18 Online:2022-03-20 Published:2022-02-22
  • Contact: Zhonghua Tang E-mail:tangzh@nefu.edu.cn
  • About author:Song Ying(1995—),femal,master,engaged in the physiological and ecological research of medicinal plants.
  • Supported by:
    Ministry of science and technology basic work special project(2019FY100505);Science and technology promotion project of State Forestry Administration(201912);Biosafety and genetic resource management project(KJZXZH202001)

摘要:

利用GC-MS技术对刺五加(Acanthopanax senticosus(Rupr. et Maxim.) Harms)和短梗五加(Acanthopanax sessiliflorus(Rupr. et Maxim.) Seem)叶片初级代谢产物进行了代谢组学分析,运用PCA和OPLS-DA方法分析后,将刺五加和短梗五加叶片划分为3个发育时期:生长期(Growth period)、旺盛期(Exuberant period)、凋落期(Autumn period)。刺五加叶片3个时期筛选出糖类、氨基酸、有机酸、脂肪酸、多元醇等共53个差异化合物,短梗五加叶片3个时期筛选出糖类、氨基酸、有机酸、脂肪酸、多元醇等共51个差异化合物。进一步分析表明,刺五加和短梗五加叶片在旺盛期和凋落期之间差异化合物最多,其中刺五加主要是糖类、有机酸;短梗五加则除了上述两类化合物外,还包括脂肪酸、多胺类化合物。两者作为同属植物,在差异化合物的组成类型方面具有较高的相似性。结果初步揭示了2种同属药用植物叶片不同时期初级代谢物的积累模式,为2种植物叶片利用提供理论基础。

关键词: 刺五加, 短梗五加, 代谢组学, 多元统计分析

Abstract:

Metabolomics analysis of Acanthopanax senticosus(Rupr. et Maxim.)Harms and Acanthopanax sessiliflorus(Rupr. et Maxim.)Seem leaves was performed using GC-MS technology. With analysis of PCA and OPLS-DA, the leaves of A. senticosus and A. sessiliflorus were divided into three developmental periods: growth period, exuberant period, and autumn period. A total of 53 differential compounds such as sugars, amino acids, organic acids, fatty acids and polyols were filtered from the leaves of A. senticosus at three periods. A total of 51 differential compounds such as sugars, amino acids, organic acids, fatty acids and polyols were filtered from the leaves of A. sessiliflorus at three periods. Further analysis showed that the leaves of A. senticosus and A. sessiliflorus had the most differential compounds in the exuberant period and autumn period. Among these differential compounds, the leaves of A. senticosus mainly included sugars and organic acids. The leaves of A. sessiliflorus also included fatty acids and polyamine compounds except for sugars and organic acids. The results initially reveal the accumulation patterns of primary metabolites in the two medicinal plants from the same genus at different periods, and provid a theoretical basis for the leaf utilization of the two plants.

Key words: Acanthopanax senticosus, Acanthopanax sessiliflorus, metabolomics, multivariate statistical analysis

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