2个慈竹NAC转录因子的克隆与组织表达分析

doi:10.7525/j.issn.1673-5102.2016.06.013

植物研究 ›› 2016, Vol. 36 ›› Issue (6): 895-901.doi: 10.7525/j.issn.1673-5102.2016.06.013

2个慈竹NAC转录因子的克隆与组织表达分析

李会萍^1,2, 黎帮勇^1,2, 胡尚连^1,2, 曹颖^1,2

1. 西南科技大学植物细胞工程实验室, 绵阳 621010;
2. 四川省生物质资源利用与改性工程技术研究中心, 绵阳 621010

收稿日期:2016-08-30 出版日期:2016-11-15 发布日期:2016-11-16
通讯作者: 胡尚连,E-mail:hushanglian@126.com E-mail:hushanglian@126.com
作者简介:李会萍(1991-),女,硕士研究生,从事植物遗传与品种改良研究。
基金资助:
国家自然科学基金（31400257，31400333）；四川省“十三五”重点公关资助项目（16ZS212301）；四川省生物质资源利用与改性工程技术研究中心基金资助（12zxsk07，13zxsk01）；西南科技大学研究生创新基金资助项目（16ycx069）

Cloning and Expression Analysis of Two NAC Transcription Factors in Bambusa emeiensis

LI Hui-Ping^1,2, LI Bang-Yong^1,2, HU Shang-Lian^1,2, CAO Ying^1,2

1. Lab of Plant Cell Engineering Southwest University of Science and Technology, Mianyang 621010;
2. Engineering Research Center for Biomass Resource Utilization and Modification of Sichuan Province, Mianyang 621010

Received:2016-08-30 Online:2016-11-15 Published:2016-11-16
Supported by:
National Natural Science Foundation of China(Nos.31400257,31400333); Breeding Program Fund Project by the 13th Five-Year Plan of Sichuan Province(Nos:16ZS212301); Fund of Engineering Research Center for Biomass Resource Utilizaiton and Modification of Sichuan Province(Nos.12zxsk07,13zxsk01); Graduate innovation fund of Southwest University of Science and Technology(Nos.16ycx069)

摘要/Abstract

摘要： 以慈竹转录组数据为基础，结合毛竹基因组数据，采用同源克隆技术从慈竹中克隆到两个NAC基因BeNAC1（GenBank注册号：KU550706）和BeNAC2（GenBank注册号：KU821586），分别编码313和389个氨基酸残基。蛋白功能域预测和保守结构域多重对比分析表明，BeNAC1和BeNAC2基因均含有NAM保守域，其模拟的蛋白质三维结构与已知晶体结构的水稻模型蛋白高度相似。亚细胞定位预测分析显示，BeNAC1主要集中在细胞质中；BeNAC2则主要集中在细胞核中。表达分析表明，BeNAC1和BeNAC2具有相似的组织特异性表达，在茎中表达量均明显高于笋和叶片，且BeNAC1在除笋之外各组织中的表达量均显著高于BeNAC2。

关键词: 慈竹, NAC转录因子, 克隆, 生物信息学分析, 组织表达模式

Abstract: Based on the transcriptome database of Bambusa emeiensis and genome information of Phyllostachys heterocycla, the two NAC genes were cloned by homology cloning technology from the shoots of B.emeiensis, named as BeNAC1(GenBank:KU550706) and BeNAC2(GenBank:KU821586), encoding 313 and 389 amino acids, respectively. By protein function prediction and conserved domain multiple alignment, both of BeNAC1 and BeNAC2 contained NAM conserved region. By protein structure prediction, the putatived three-dimensional structure of BeNAC1 and BeNAC2 protein was similar to a rice protein with known crystal structure. By subcellular localization prediction analysis, BeNAC1 was mainly concentrated in the cytoplasm, and BeNAC2 was mainly concentrated in the nucleus. Tissue expression pattern analysis showed that BeNAC1 and BeNAC2 displayed similar tissue-specific, both having the highest expression on stems, whereas in addition to the bamboo shoots, the expression of BeNAC1 in each tissue tested was higher than that of BeNAC2.

Key words: Bambusa emeiensis, NAC transcription factors, clone, bioinformatics analysis, tissue expression pattern

中图分类号:

S795.5

李会萍, 黎帮勇, 胡尚连, 曹颖. 2个慈竹NAC转录因子的克隆与组织表达分析[J]. 植物研究, 2016, 36(6): 895-901.

LI Hui-Ping, LI Bang-Yong, HU Shang-Lian, CAO Ying. Cloning and Expression Analysis of Two NAC Transcription Factors in Bambusa emeiensis[J]. Bulletin of Botanical Research, 2016, 36(6): 895-901.

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2个慈竹NAC转录因子的克隆与组织表达分析

Cloning and Expression Analysis of Two NAC Transcription Factors in Bambusa emeiensis

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