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植物研究 ›› 2010, Vol. 30 ›› Issue (3): 360-364.doi: 10.7525/j.issn.1673-5102.2010.03.019

• 论文 • 上一篇    下一篇

翻白草降糖作用有效部位的研究

孙海峰;杨婷;郭冷秋;曹思思;郭雪莹   

  1. 黑龙江中医药大学药学院,哈尔滨 150040
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-05-20 发布日期:2010-05-20
  • 基金资助:
     

The Effective Part of Potentilla discolor for Its Hypoglycemic Action

SUN Hai-Feng;YANG Ting;GUO Leng-Qiu;CAO Si-Si;GUO Xue-Ying   

  1. Pharmacy College,Heilongjiang University of Chinese Medicine,Harbin 150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-05-20 Published:2010-05-20
  • Supported by:
     

摘要: 采用系统溶剂提取法和大孔树脂富集技术对翻白草进行分部位提取;建立小鼠糖尿病模型,对各提取物进行降血糖药效学研究;用紫外分光光度法测定翻白草不同提取物中总黄酮的含量,以研究翻白草降糖作用的有效部位。结果表明:翻白草50%醇提物正丁醇萃取物和大孔树脂70%乙醇洗脱部位能明显地降低糖尿病模型小鼠的血糖。翻白草不同提取物中以50%醇提物正丁醇萃取物的总黄酮含量最高,翻白草大孔树脂70%乙醇洗脱部位总黄酮含量达到58.25%。经分析认为,正丁醇提取物和翻白草大孔树脂70%乙醇洗脱部位为翻白草降血糖有效部位,且其中的黄酮类物质是翻白草的降血糖活性成分。

关键词: 翻白草, 糖尿病, 有效部位, 黄酮

Abstract: The extracts of Potentilla discolor Bunge. were extrated by different solvents, and enriched by macroporous resins.The pharmacological effect of the different extracts from P.discolor Bunge.was tested by hyperglycemic model mice.The total flavonoids in different extracts from P.discolor Bge. were determined by UV-spectrophotometry. The result showed that the n-BuOH fractions of 50% alcohol extracts and 70% ethanolic elutes from a macroporous resins had obvious hypoglycemic action. It could notably reduce the blood glucose of hyperglycemic mice. The content of total flavonoids of the n-BuOH fractions of 50% alcohol extracts is the highest, and the contents in 70% ethanolic elutes from macroporous resins reaches 58.25%. Therefore, the n-BuOH fractions of 50% alcohol extracts and 70% ethanolic elutes from macroporous resins are the effective parts of P.discolor Bunge. for its hypoglycemic action. Flavonoids maybe the active constituents with the hypoglycemic action in P.discolor Bunge.

Key words: Potentilla discolor Bunge., diabetes, effective parts, flavonoids

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