黑暗条件下褪黑素对栀子叶片类黄酮含量及相关基因表达水平的影响

doi:10.7525/j.issn.1673-5102.2018.04.010

摘要/Abstract

摘要： 以栀子叶片为材料，通过转录组测序与液相色谱技术来研究黑暗条件下褪黑素对栀子叶片类黄酮含量及相关基因表达水平的影响，结果显示褪黑素不仅延缓了栀子叶片的黄化，而且主要通过抑制类黄酮等生物合成途径上的相关基因表达，进而减少类黄酮积累来延缓叶片黄化，这对黑暗胁迫下延缓栀子叶片的黄化提供实际指导，也为利用褪黑素对叶片黄化的控制与延缓提供了借鉴与参考。

关键词: 栀子, 黑暗胁迫, 褪黑素, 类黄酮, 转录组, 液相色谱

Abstract: Gardenia leaves were used as materials to study the effect of melatonin on flavonoid content and related gene expression in Gardenia leaves under dark conditions by transcriptome sequencing and liquid chromatography. The melatonin not only delayed the senescence of Gardenia, but also delayed the yellowing of leaves by reducing the accumulation of flavonoids through inhibiting the expression of related genes such as flavonoids biosynthetic pathways. Our experiment provided practical guidance for retarding the yellowing of Gardenia leaves under dark stress, and the reference to the use of melatonin to control and delay the yellowing of leaves.

Key words: Gardenia, dark stress, melatonin, flavonoids, transcriptome, HPLC

中图分类号:

Q949.781.1

王荣, 成梦琳, 刘慧娜, 赵大球, 陶俊. 黑暗条件下褪黑素对栀子叶片类黄酮含量及相关基因表达水平的影响[J]. 植物研究, 2018, 38(4): 559-567.

WANG Rong, CHENG Meng-Lin, LIU Hui-Na, ZHAO Da-Qiu, TAO Jun. Effects of Melatonin on Flavonoids Content and Related Gene Expression Levels of Gardenia under Dark Conditions[J]. Bulletin of Botanical Research, 2018, 38(4): 559-567.

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