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植物研究 ›› 2020, Vol. 40 ›› Issue (6): 886-896.doi: 10.7525/j.issn.1673-5102.2020.06.011

• 研究报告 • 上一篇    

基于转录组测序的细风轮花青素合成途径及关键酶基因分析

赵历强1,2, 单春苗1,2, 张声祥1,2, 施圆圆1,2, 马克龙3, 吴家文2,4,5()   

  1. 1.安徽中医药大学研究生院,合肥 230012
    2.安徽中医药大学科研实验中心、新安医学教育部重点实验室,合肥 230038
    3.安徽中医药大学中西医结合学院,合肥 230012
    4.安徽道地中药材品质提升协同创新中心,合肥 230012
    5.安徽省中医药科学院,合肥 230012
  • 收稿日期:2019-11-19 出版日期:2020-11-20 发布日期:2020-11-04
  • 通讯作者: 吴家文 E-mail:wujiawen@ahtcm.edu.cn
  • 作者简介:赵历强(1995—),男,硕士研究生,主要从事转录组学研究。
  • 基金资助:
    安徽高校自然科学研究重大项目(KJ2018ZD028);名贵中药资源可持续利用能力建设项目(2060302);国家自然科学基金(81874431);国家级大学生创新训练项目(201810369050)

Identification of Key Enzyme Genes Involved in Anthocyanin Synthesis pathway in Clinopodium gracile by Transcriptome Analysis

Li-Qiang ZHAO1,2, Chun-Miao SHAN1,2, Sheng-Xiang ZHANG1,2, Yuan-Yuan SHI1,2, Ke-Long MA3, Jia-Wen WU2,4,5()   

  1. 1.Graduate School,Anhui University of Chinese Medicine,Hefei 230012
    2.Key Laboratory of Xin’an Medicine,Ministry of Education,Anhui University of Chinese Medicine,Hefei 230038
    3.Clinical College of Integrated Traditional Chinese and Western Medicine,Anhui University of Chinese Medicine,Hefei 230012
    4.Synergetic Innovation Center of Anhui Authentic Chinese Medicine Quality Improvement,Hefei 230012
    5.Anhui Academy of Chinese Medicine,Hefei 230012
  • Received:2019-11-19 Online:2020-11-20 Published:2020-11-04
  • Contact: Jia-Wen WU E-mail:wujiawen@ahtcm.edu.cn
  • About author:ZHAO Li-Qiang(1995—),male,master degree candidate,mainly engaged in transcriptomics research.
  • Supported by:
    The Natural Science Research Grant of Higher Education of Anhui Province(KJ2018ZD028);Sustainable Utilization Project of Chinese Medicine Resources(2060302);National Natural Science Foundation of China(81874431);National College Students’ Innovation and entrepreneurship Training Program,China(201810369050)

摘要:

为进一步理解细风轮花青素合成途径,本研究利用华大基因BGISEQ-500平台对细风轮中的根、茎、叶、花4个组织进行了转录组测序,从头组装后得到128 856个Unigene。KEGG通路表明有40个Unigene编码了细风轮的花青素生物合成途径中6个关键酶。我们对其中的关键酶DFR(二氢黄酮醇还原酶)进行同源比对和空间结构模拟,结果显示DFR序列和结构均具有良好的保守性,且具有高度保守的NAD+结合位点,其二级结构主要由α螺旋和β折叠组成,在空间上α螺旋包裹着β折叠,形成“夹心饼干”样结构。

关键词: 细风轮, 花青素, 转录组, 单基因, 二氢黄酮醇还原酶

Abstract:

In order to further understand the pathway of anthocyanin synthesis, the four tissues of C.gracile, namely, root, stem, leaf and flower, were sequenced using BGISEQ-500 platform. 128 856 unigenes were assembled and 40 unigenes encoding six key enzymes involved in the anthocyanin biosynthesis were identified. Homologous alignment and structure simulation of bifunctional dihydroflavonol 4-reductase(DFRs) were carried out, which showed that they have well-conserved sequences, spatial structures and NAD+ binding sites. The DFR structure model showed a tetrameric structure with Rossmann-like α-helixes/β-sheets/α-helixes sandwich fold.

Key words: Clinopodium gracile, anthocyanin, transcriptome, unigene, DFR

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