Cloning and Functional Analysis of Heat Shock Protein HbHSP90.8-1 from Hevea brasiliensis Müll. Arg.

doi:10.7525/j.issn.1673-5102.2022.05.012

Abstract

Abstract:

Powdery mildew is one of the major leaf diseases in growth of rubber tree. Heat shock protein 90（HSP90） molecular chaperone plays an important role in plant stress resistance. To study the structure of the rubber tree HSP90 family members and their function in powdery mildew resistance， HbHSP90.8-1 was cloned from rubber tree variety Reyan73397 leaf using PCR technology and its structure and function were analyzed by bioinformatics method. The results showed that the cDNA sequence of HbHSP90.8-1 was 2 844 bp in full length， 2 454 bp open reading frame（ORF） and encoded 817 amino acids. HbHSP90.8-1 encoded a stable hydrophilic protein with signal peptide， no transmembrane structure and predicted positioning on endoplasmic reticulum， contained HSP90 superfamily and HATPase superfamily domains. Phylogenetic analysis showed that HbHSP90.8-1 was closely related to MeHSP90， and classified into Jatropha JcHSP90 and Castor RcHSP90. The qRT-PCR analysis showed that HbHSP90.8-1 was expressed in different tissues of rubber tree， and its expression level was the highest in latex. The expression of HbHSP90.8-1 was significantly expressed and up-regulated in leaves treated with Oidium heveae infection， H₂O₂， ABA and ETH respectively. However， under the treatment of MeJA and SA hormone， the expression of HbHSP90.8-1 showed a downward trend. The study suggested that HbHSP90.8-1 related to the powdery mildew resistance and the hormone signal transduction pathway related to plant disease resistance， and provided a technical guidance for the subsequent study on the molecular regulation mechanism of HbHSP90.8-1 in the powdery mildew resistance in rubber tree.

Key words: rubber tree, powdery mildew, HbHSP90.8-1, bioinformatics, expression analysis

CLC Number:

S664.9

Mingyang LIU, Huaxing XIAO, Lifeng WANG, Xiaoxu LIANG, Yu ZHANG, Meng WANG. Cloning and Functional Analysis of Heat Shock Protein HbHSP90.8-1 from Hevea brasiliensis Müll. Arg.[J]. Bulletin of Botanical Research, 2022, 42(5): 811-820.

Figures/Tables 7

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名称 Name	网址 Website
NCBI ORF Finder	www.ncbi.nlm.nih.gov/orffinder/
ExPASy ProtParam tool	web.expasy.org/protparam/
TMHMM Server v.2.0	www.cbs.dtu.dk/services/TMHMM-2.0/
DeepLoc1.0	www.cbs.dtu.dk/services/DeepLoc/
SignalP-5.0 Server	http：//www.cbs.dtu.dk/services/SignalP/
SWISS-MODEL	swissmodel.expasy.org/
SOPMA	https：//npsa-prabi.ibcp.fr/cgi-bin/secpred_sopma.pl
NCBI smart BLAST	blast.ncbi.nlm.nih.gov/Blast.cgi
MEME	meme-suite.org/tools/meme

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