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Bulletin of Botanical Research ›› 2016, Vol. 36 ›› Issue (3): 401-408.doi: 10.7525/j.issn.1673-5102.2016.03.013

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Transformation of Mirabilis jalapa L. with otsB Gene in E.coli Driven by rd29A Promoter from Arabidopsis thaliana

LU Yu-Jian1,2,3, ZHANG Han-Jie3, HAN Wen-Yu2, SHEN Zhi-Qiang1,4   

  1. 1. Postdoctoral Programme, Binzhou Animal Science & Veterinary Medicine Academy, Binzhou 256600;
    2. Postdoctoral Programme, Jilin University, Changchun 130062;
    3. Shandong Provincial Engineering and Technology Research Center for Wild Plant Resources Development and Application of Yellow River Delta, Department of Life Sciences, Binzhou University, Binzhou 256603;
    4. Binzhou Animal Science & Veterinary Medicine Academy, Binzhou 256600
  • Received:2015-11-19 Online:2016-05-15 Published:2016-05-20

Abstract: Mirabilis jalapa L. is a perennial herb of Nyctaginaceae. Not only is it a high-value ornamental flowering and greening plants, but also can be used as medicine. M.jalapa L. has good bioremediation function. M.jalapa L. was used as experimental material, and the perfect regeneration system was initially established. The rd29A promoter of Arabidopsis thaliana and otsB gene in E.coli were cloned by PCR respectively. Afterwards, the rd29A promoter and otsB gene were ligated into plasmid p2300-GFP, which would lead to the construction of p2300-rd29A pro-otsB expression vector. Then, the expression vector was introduced into the cells of M.jalapa L. by Agrobacterium-mediated method. The genetic transformation results showed that when the concentration of Agrobacterium was OD600=0.5, the time of infection of mature embryo or nodal stem segments was 60 min, and the co-culture time was 2 d, the transformation efficiency of M.jalapa L. was the highest. By PCR detection, otsB gene was successfully integrated into the genome of M.jalapa L., and the efficient transcription could be performed.

Key words: Mirabilis jalapa L., rd29A promoter, otsB gene, Agrobacterium, genetic transformation

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