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Bulletin of Botanical Research ›› 2017, Vol. 37 ›› Issue (4): 542-548.doi: 10.7525/j.issn.1673-5102.2017.04.009

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The Comparative study of Callus and Direct Differation Regenaration System of 84K Poplar

WANG Li-Na, WANG Yu-Cheng, YANG Chuan-Ping   

  1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin 150040
  • Received:2017-02-02 Online:2017-07-15 Published:2017-07-22
  • Supported by:
    Fundamental Research Funds for the Central Universities(2572016BA02);Harbin applied technology research and development project(2016RAQXJ065)

Abstract: High-frequent transformation and strong regeneration ability of plants are prerequisites to the establishment of good plant transformation receptor system, which is the foundation for plant genetic transformation. Taking 84K poplar leaves as study material, this experiment studies the influence of different types and concentrations of plant hormones to its dedifferentiation and re-differentiation under different cultivation conditions, and sets two mature plant tissue cultivation regeneration system. Based on above,here establishes genetic transformation system of adventitious buds and callus pathway, respectively as callus regeneration system and regeneration system of direct differentiation. Then through conducting comparative analysis of two tissue culture system using PCK1 and PCK2, here summarizes the advantages and disadvantages of two methods.

Key words: Poplar 84K, callus, direct differation, genetic transformation

CLC Number: