Molecular Cloning and Genetic Transformation Analysis of an Apple Auxin Influx Transporter Gene MdAUX1 in Arabidopsis thaliana and Tobacco

doi:10.7525/j.issn.1673-5102.2016.06.014

Abstract

Abstract: Auxin has been identified to play critical roles in regulating plant growth and development. The polar transport of auxin is regulated by auxin transporters. In this study, an auxin influx transporter gene named MdAUX1 was cloned from Malus×domestic‘Royal Gala’. Sequence analysis showed that the length of MdAUX1 gene was 1 443 bp, which encoded 480 amino acids. Amino acid sequence alignment and phylogenetic tree analysis showed that the AUX1 had a high sequence conservation among different species, and apple MdAUX1 exhibited the highest sequence similarity with Prunus avium PaAUX1. RT-PCR analysis demonstrated the MdAUX1 gene was mainly expressed in stem. We generated transgenic Arabidopsis and tobacco plants expressing the apple MdAUX1 by Agrobacterium-mediated genetic transformation. Ectopic expression of MdAUX1 in Arabidopsis exhibited the phenotype of inhibition of primary root elongation and increase lateral root number. Furthermore, there was no significant inhibition on primary root elongation phenotype when treated with the NAA. Overexpressing MdAUX1 in tobacco could significantly promote the growth of aerial parts of plant. These results suggested that MdAUX1 played a vital role in plant growth and development.

Key words: apple, auxin influx transporters, MdAUX1, genetic transformation

CLC Number:

S661.1

AN Jian-Ping, WANG Xiao-Fei, LIU Xin, LI Hao-Hao, YOU Chun-Xiang, HAO Yu-Jin. Molecular Cloning and Genetic Transformation Analysis of an Apple Auxin Influx Transporter Gene MdAUX1 in Arabidopsis thaliana and Tobacco[J]. Bulletin of Botanical Research, 2016, 36(6): 902-908.

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