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Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (3): 394-402.doi: 10.7525/j.issn.1673-5102.2022.03.009

• Genetic and Breeding • Previous Articles     Next Articles

Identification of Broussonetia papyrifera Transcription Factor BpbZIP1 and Analysis of Its Response to Cadmium Stress

Sisi CHEN, Muhong XIE, Maokai CUI, Wenkai LI, Zhenggang XU, Caixia JIA, Guiyan YANG()   

  1. Key Laboratory of Economic Plant Resources Development and Utilization in Shaanxi Province,College of Forestry,Northwest A & F University,Yangling 712100
  • Received:2021-03-29 Online:2022-05-20 Published:2022-05-20
  • Contact: Guiyan YANG E-mail:yangguiyan@nwsuaf.edu.cn
  • About author:CHEN Sisi(1996—),female,master,mainly engaged in economic forest breeding.
  • Supported by:
    Financial Grant from the China Postdoctoral Science Foundation(2020M683592);National Natural Science Foundation of China(31700332)


Heavy metal pollution not only affected the effective area of the soil, restricted the distribution of vegetation, but also harmed the food chain and human health, and cadmium(Cd) pollution was particularly prominent. It was urgent to select plants with strong heavy metal tolerance for bioremediation in tailings areas. For Broussonetia papyrifera was a pioneer tree species in heavy metals polluted soil, In order to explore the molecular mechanism of B. papyrifera in response to heavy metal stress, a basic leucine zipper(bZIP) transcription factor(named BpbZIP1) from B. papyrifera was cloned, and its basic biological information, Cd stress response and the function of transformed yeast were analyzed to predict the role of BpbZIP1 in response to Cd. The results showed that the open reading frame of BpbZIP1 was 1 713 bp, the encoded protein contained 570 amino acids, the molecular weight was 62 902.38 Da, and the theoretical isoelectric point was 4.62. It had a close evolutionary relationship with Arabidopsis thaliana AtbZIP1. Under 150 μmol·L-1 CdCl2 treatment, the BpbZIP1 in B. papyrifera could be induced differently, and the expression of BpbZIP1 in roots was 17.4-fold higher than that in controls at 3 h after induction. Transformation of BpbZIP1 into yeast could significantly improve Cd resistance of transgenic yeasts. When the CdCl2 concentration was higher than 0.6%, the growth vigor of transgenic yeast was 1.54-1.71-fold higher than that in controls. These results suggested that BpbZIP1 gene could positively respond to Cd stress, and its expression benefited Cd stress tolerance. BpbZIP1 was an important candidate in response to Cd stress of B. papyrifera.

Key words: Broussonetia papyrifera, Cd stress, bZIP transcription factor, gene expression, yeast transformation

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