Cloning and Bioinformatics Analysis of CbuATX1,CbuATX1-like and CbuATX2 Genes from Catalpa bungei

doi:10.7525/j.issn.1673-5102.2022.01.006

Abstract

Abstract:

Flowering is a complex life process that is regulated by both genetic factors and environmental factors in higher plants. It is a symbol of plants genetic and reproductive ability after it enters into reproductive growth. The genes of CbuATX1， CbuATX1-like and CbuATX2 were cloned respectively by PCR with cDNA as a template from Catalpa bungei flower bud， and the related bioinformatics software was used to predict the protein structure and subcellular location of these three genes respectively. The three genes promoter sequence were analyzed in cis-acting elements， and the expression levels of the three genes in different stages of C. bungei flower bud development were detected by qRT-PCR. The results showed that the CDS of CbuATX1 was 726 bp， encoding 241 amino acids with transmembrane transport structure. The protein was belonged to the HMA protein family and closely related to Sesamum indicum and Beta vulgaris. The CDS of CbuATX1-like was 801 bp. encoding 266 amino acids with transmembrane transport structure. The protein was belonged to the HMA protein family， which was closely related to Daucus carota and Olea europaea. The CDS of CbuATX2 was 1 554 bp， encoding 517 amino acids with no transmembrane transport structure. The protein was belonged to the PWWP protein family and closely related to Erythranthe guttatus. The promoters of three genes contained several basic elements of eukaryotic promoters respectively， such as CAAT box and TATA box. In addition， they also contained light response factors， low temperature response factors and biological activity elements. The results of qRT-PCR showed that the expression levels of the three genes were significantly different in different developmental stages. It was hoped to further elaborate the flowering characteristics of C. bungei， and provided theoretical support for the flowering mechanism and directional genetic improvement of C. bungei.

Key words: Catalpa bungei, CbuATX, clone, flower, bioinformatics analysis

CLC Number:

S792.26

Jiaming Zhao, Erqin Fan, Yi Liu, Zhi Wang, Junhui Wang, Guanzheng Qu. Cloning and Bioinformatics Analysis of CbuATX1,CbuATX1-like and CbuATX2 Genes from Catalpa bungei[J]. Bulletin of Botanical Research, 2022, 42(1): 47-61.

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