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Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (1): 106-116.doi: 10.7525/j.issn.1673-5102.2020.01.015

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Structural and Functional Analysis of R2R3 MYB Transcription Factor HbMYB88

FAN Song-Le1,2, WANG Ji-Kun2, XIE Gui-Shui2, WANG Meng1, WANG Li-Feng2   

  1. 1. Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, College of Tropical Crop, Hainan University, Haikou 570228;
    2. Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs, P. R. China;State Key Laboratory Incubation Base for Cultivation & Physiology of Tropical Crops;Danzhou Investigation & Experiment Station of Tropical Crops, Ministry of Agriculture and Rural Affairs, P. R. China;Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101
  • Received:2019-06-04 Online:2020-01-05 Published:2020-02-21
  • Supported by:
    National Natural Science Foundation of China(31570591);Earmarked fund for China Agriculture Research System(CARS-33-ZP1);Innovative scientific research project for master student of colleges and universities in hainan province(Hys2019-142)

Abstract: The R2R3-MYB transcription factors are involved in biological processes, such as plant growth and development, hormone signaling transduction, and stress response, etc. To explore the structure and functions of the MYB family members in the rubber tree, we cloned the full-length cDNA of HbMYB88 from the leave of rubber tree Reyan 73397(RY73397). It is 1 848 bp in length, containing a 1 440 bp ORF which encodes 479 amino acid residues. The deduced amino acid sequence of HbMYB88 contains two SANT conserved domains, has HTH tertiary structure, share highly similarity to Arabidopsis AtMYB88, AtMYB124. AtMYB88 and AtMYB124, not divided into subfamilies, are related to drought stress response, etc. By qRT-PCR analysis, HbMYB88 was mainly expressed in the stem and flower of rubber tree, whilst its expressions in root, leaf, bark and latex were extremely low. The expression level of HbMYB88 in tissue culture seedling leave was significantly upregulated under the treatment of hydrogen peroxide(H2O2), and abscisic acid(ABA). These indicated that HbMYB88 has a relationship with stress response such as drought in rubber trees, which providing a foundation for further study of its structure and function.

Key words: Hevea brasiliensis, HbMYB88, abscisic acid, gene expression

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