R2R3型MYB转录因子HbMYB88结构和功能分析

doi:10.7525/j.issn.1673-5102.2020.01.015

植物研究 ›› 2020, Vol. 40 ›› Issue (1): 106-116.doi: 10.7525/j.issn.1673-5102.2020.01.015

R2R3型MYB转录因子HbMYB88结构和功能分析

樊松乐^1,2, 王纪坤², 谢贵水², 王萌¹, 王立丰²

1. 海南大学热带作物学院, 海南省热带生物资源可持续利用重点实验室, 海口 570228;
2. 农业农村部橡胶树生物学与遗传资源利用重点实验室;省部共建国家重点实验室培育基地——海南省热带作物栽培生理学重点实验室;农业农村部儋州热带作物科学观测实验站;中国热带农业科学院橡胶研究所, 海口 571101

收稿日期:2019-06-04 出版日期:2020-01-05 发布日期:2020-02-21
通讯作者: 王立丰 E-mail:lfwang@catas.cn
作者简介:樊松乐(1993-),男,硕士研究生,主要从事农艺与种业研究。
基金资助:
国家自然科学基金（31570591）；现代农业产业技术体系建设专项资金（CARS-33-ZP1）；海南省普通高等学校研究生创新科研课题（Hys2019-142）

Structural and Functional Analysis of R2R3 MYB Transcription Factor HbMYB88

FAN Song-Le^1,2, WANG Ji-Kun², XIE Gui-Shui², WANG Meng¹, WANG Li-Feng²

1. Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, College of Tropical Crop, Hainan University, Haikou 570228;
2. Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs, P. R. China;State Key Laboratory Incubation Base for Cultivation & Physiology of Tropical Crops;Danzhou Investigation & Experiment Station of Tropical Crops, Ministry of Agriculture and Rural Affairs, P. R. China;Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101

Received:2019-06-04 Online:2020-01-05 Published:2020-02-21
Supported by:
National Natural Science Foundation of China(31570591);Earmarked fund for China Agriculture Research System(CARS-33-ZP1);Innovative scientific research project for master student of colleges and universities in hainan province(Hys2019-142)

摘要/Abstract

摘要： R2R3-MYB转录因子参与植物生长发育、激素信号传导和逆境响应等生物过程。为了揭示橡胶树MYB家族成员结构与功能，从橡胶树热研73397（RY73397）叶片中克隆HbMYB88的cDNA全长。该基因长为1 848 bp，含1 440 bp的ORF，编码479个氨基酸。HbMYB88蛋白序列包含2个SANT保守结构域，存在HTH三级结构，与拟南芥AtMYB88、AtMYB124具有高度相似性。AtMYB88、AtMYB124与干旱等逆境相关且未划分亚族。qRT-PCR分析发现HbMYB88在橡胶树茎和花中表达量高，在根、叶片、树皮和胶乳中表达量极低。热研73397组培苗叶片喷施过氧化氢（H₂O₂）和脱落酸（ABA）等处理后，HbMYB88表达量受诱导显著上调表达。表明HbMYB88与橡胶树抗旱等逆境反应有关，为深入研究其结构和功能打下基础。

关键词: 巴西橡胶树, HbMYB88, 脱落酸, 基因表达

Abstract: The R2R3-MYB transcription factors are involved in biological processes, such as plant growth and development, hormone signaling transduction, and stress response, etc. To explore the structure and functions of the MYB family members in the rubber tree, we cloned the full-length cDNA of HbMYB88 from the leave of rubber tree Reyan 73397(RY73397). It is 1 848 bp in length, containing a 1 440 bp ORF which encodes 479 amino acid residues. The deduced amino acid sequence of HbMYB88 contains two SANT conserved domains, has HTH tertiary structure, share highly similarity to Arabidopsis AtMYB88, AtMYB124. AtMYB88 and AtMYB124, not divided into subfamilies, are related to drought stress response, etc. By qRT-PCR analysis, HbMYB88 was mainly expressed in the stem and flower of rubber tree, whilst its expressions in root, leaf, bark and latex were extremely low. The expression level of HbMYB88 in tissue culture seedling leave was significantly upregulated under the treatment of hydrogen peroxide(H₂O₂), and abscisic acid(ABA). These indicated that HbMYB88 has a relationship with stress response such as drought in rubber trees, which providing a foundation for further study of its structure and function.

Key words: Hevea brasiliensis, HbMYB88, abscisic acid, gene expression

中图分类号:

S794.1

樊松乐, 王纪坤, 谢贵水, 王萌, 王立丰. R2R3型MYB转录因子HbMYB88结构和功能分析[J]. 植物研究, 2020, 40(1): 106-116.

FAN Song-Le, WANG Ji-Kun, XIE Gui-Shui, WANG Meng, WANG Li-Feng. Structural and Functional Analysis of R2R3 MYB Transcription Factor HbMYB88[J]. Bulletin of Botanical Research, 2020, 40(1): 106-116.

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R2R3型MYB转录因子HbMYB88结构和功能分析

Structural and Functional Analysis of R2R3 MYB Transcription Factor HbMYB88

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