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植物研究 ›› 2020, Vol. 40 ›› Issue (4): 523-529.doi: 10.7525/j.issn.1673-5102.2020.04.006

• 研究报告 • 上一篇    

温水浸种对杜仲种子萌发过程中保护酶活性及内源激素含量变化的影响

王宁1, 董莹莹2, 袁美丽3, 王亚楠1, 曾胜蕴1   

  1. 1. 河南科技大学林学院, 洛阳 471023;
    2. 洛阳市城市管理局, 洛阳 471002;
    3. 洛阳市隋唐城遗址植物园, 洛阳 471002
  • 收稿日期:2019-09-29 发布日期:2020-06-12
  • 作者简介:王宁(1979-),男,博士,讲师,主要从事园林植物逆境生理研究。
  • 基金资助:
    河南省自然科学基金项目(182300410092)

Effect of Warm Water Soaking on Protective Enzyme Activity and Endogenous Hormones Content of Eucommia ulmoides Seed During Different Stages of Germination

WANG Ning1, DONG Ying-Ying2, YUAN Mei-Li3, WANG Ya-Nan1, ZENG Sheng-Yun1   

  1. 1. College of Forestry, Henan University of Science and Technology, Luoyang 471023;
    2. Luoyang Urban Management Bureau, Luoyang 471002;
    3. The Sui & Tang Dynasties Relics Botanic Garden of Luoyang, Luoyang 471002
  • Received:2019-09-29 Published:2020-06-12
  • Supported by:
    The Natural Science Foundation of Henan Province(182300410092)

摘要: 探讨杜仲(Eucommia ulmoides Oliver)种子萌发过程中保护酶活性及内源激素含量的变化,为其种子破眠及萌发不同时期采取有效处理措施,从而提高出苗率提供参考。利用室温(CK)、40℃、50℃、60℃和70℃不同水温,探讨温水浸种对杜仲种子萌发的影响。种子萌发进程中,利用常规生理生化方法测定种子内超氧化物歧化酶(SOD)、过氧化物酶(POD)活性和丙二醛(MDA)含量,以及酶联免疫法测定赤霉素(GA3)、吲哚乙酸(IAA)、脱落酸(ABA)和玉米素核苷(ZR)含量。结果表明:①温水浸种处理能提高了杜仲种子的发芽率及发芽势,其中以50℃温水处理最为显著。②与室温浸种处理相比,50℃温水浸种处理提高了种子内SOD及POD活性,减轻了质膜氧化损伤程度,致使MDA含量的持续下降,从而促使种子朝向有利于萌发的方向发展。此外,50℃温水浸种处理在提高种子内促进生长内源GA3、IAA及ZR含量的同时,并降低了抑制生长的内源ABA含量,致使GA3/ABA、IAA/ABA和ZR/ABA值均明显高于室内浸种处理,最终促进了杜仲种子的萌发。

关键词: 杜仲, 萌发阶段, 超氧化物歧化酶, 内源激素, 丙二醛, 脱落酸

Abstract: The experiment was conducted to investigate the regulation mechanism of enzymes and endogenous hormones of different stages of Eucommia ulmoides Oliver seed germination, aiming to provide scientific basis for taking effective treatment measures during the different stages of seed dormancy and germination to increase seedling emergence rate. The effects of warm water soaking on the germination of E.ulmoides seeds were studied with room temperature(CK), 40℃, 50℃, 60℃ and 70℃. And the activities of superoxide dismutase(SOD), peroxidase(POD), and malondialdehyde(MDA) content were determined by routine physiological and biochemical methods. Levels of 4 endogenous hormones, such as gibberellin(GA3), indole acetic acid(IAA), zeatin riboside(ZR) and abscisic acid(ABA) were quantitatively determined by enzyme-linked immunosorbent assay. The result showed that:①The seed germination rate could be increased by soaking seeds with different temperature water, and the germination rate reached its maximum under 50℃ warm water treatment. ②Compared with room temperature water treatment, the activity of SOD and POD in the seeds were increased under 50℃ warm water, and reduced the degree of cytoplasm membrane was oxidized, and the content of MDA was decreased continuously, thus promoted the development of seeds in a direction favorable for germination. In addition, treatment with 50℃ warm water promoted the growth of endogenous GA3, IAA and ZR in seeds, and decreased the content of endogenous ABA, and the values of GA3/ABA, IAA/ABA and ZR/ABA were significantly higher than those of room temperature water, which ultimately promoted the germination of E.ulmoides.

Key words: Eucommia ulmoides, germinating stage, SOD, endogenous hormones, MDA, ABA

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