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Bulletin of Botanical Research ›› 2013, Vol. 33 ›› Issue (2): 197-201.doi: 10.7525/j.issn.1673-5102.2013.02.013

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Establishment and Optimization of Genetic Transformation and Introduction of TaNHX2 Gene by Agrobacterium tumefaciens in Albizia julibrissin

ZHOU Xiang-Ming;;HAO Zhi-Yu;XIA Shi-Yun;WANG Shu;SONG Jian;CHEN Shou-Yi;LIU Zhong-Qi   

  1. 1.Tianjin Research Center of Agricultural Biotechnology, Tianjin　300384；2.National Engineering Laboratory for Tree Breeding,Beijing Forestry University,Beijing　100083；3.Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants,Ministry of Education,Beijing Forestry University,Beijing　100083；4.The National Key Lab of Plant Genomics,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing　100101
  • Received:1900-01-01 Revised:1900-01-01 Online:2013-03-20 Published:2013-03-20
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Abstract: Some factors influencing the transformation efficiency, such as the concentration and infection duration of Agrobacterium tumefaciens, pre-culture and co-culture duration, were optimized to establish a stable Agrobacterium mediated gene transformation protocol of Albizia julibrissin Duraxx. The TaNHX2 gene was transformed into A.julibrissin. Mediated by A.tumefaciens, lots of regenerated plants resistant to Kanamycin (Kan) were obtained. PCR analysis and real time PCR detection of some transgenic plants showed that the TaNHX2 gene was evidently integrated into the genome of transgenic plants and could be transcribed properly.

Key words: TaNHX2, Albizia julibrissin, genetic transformation, Agrobacterium tumefaciens

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