Isolation, Purification and Identification of Triter-penoids from Rosa roxburghii Leaves

doi:10.7525/j.issn.1673-5102.2024.06.003

Abstract

Abstract:

The R. roxburghii leaves were used as materials， and the triterpenoid chemical components were systematically isolated and purified from leaves by using methods of silica gel column chromatography， Sephadex LH-20 column chromatography， and semi HPLC， respectively. The structures of the compounds were identified through physicochemical properties and nuclear magnetic resonance spectroscopy （NMR） spectral data， and a total of 13 compounds were isolated and identified. Among them， there were two tetracyclic triterpenoid compounds， three pentacyclic triterpenoid glycosides， and eight pentacyclic triterpenoid carboxylic acid compounds， including a novel compound monomer. The structures identified were （1）β- sitosterol，（2）daucosterol，（3）2α，3α，19α-trihydroxyolean-12-en-28-O-β-D-glucopyranoside，（4）kaji-ichigoside F1，（5）rosamultin，（6）rosamultic acid，（7）new compound，（8）hyptadienic acid，（9）euscaphic acid，（10）tormentic acid，（11）corosolic acid，（12）betulinic acid， and （13）maslinic acid， respectively， and the compounds No.2， No.6， No.7 and No.8 were isolated and identified from this plant for the first time. This study clarified the triterpenoid components in R. roxburghii leaves， and provided scientific basis for the development and utilization of R. roxburghii leaf resources.

Key words: Rosa roxburghii leaves, triterpenoid, separation, structural identification

CLC Number:

O629.61

Yanhao LIU, Jiandong WANG, Yujie FU. Isolation, Purification and Identification of Triter-penoids from Rosa roxburghii Leaves[J]. Bulletin of Botanical Research, 2024, 44(6): 822-831.

Figures/Tables 16

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位置 Position	化合物1 Compound 1	化合物2 Compound 2	化合物3 Compound 3	化合物4 Compound 4	化合物5 Compound 5
1	37.26	36.83	42.19	42.98	48.06
2	31.66	29.26	66.14	66.14	68.66
3	71.81	76.74	79.37	79.37	83.90
4	42.30	39.98	38.89	38.81	38.53
5	140.77	140.44	48.90	48.78	56.00
6	121.73	122.19	18.73	22.31	19.08
7	31.91	31.41	33.07	33.51	33.54
8	31.92	31.37	40.48	40.80	40.65
9	50.14	49.60	48.27	48.64	48.66
10	36.51	36.21	38.86	38.69	39.88
11	21.09	20.60	24.26	24.15	24.60
12	39.78	38.31	123.83	128.41	128.89
13	42.33	41.85	144.36	139.29	140.70
14	56.78	56.14	42.71	42.14	42.18
15	24.31	25.43	28.77	29.48	29.38
16	28.26	28.70	27.99	26.13	26.14
17	56.06	55.43	46.48	47.69	47.89
18	11.99	11.78	44.60	54.43	54.46
19	19.83	19.09	81.03	74.08	71.26
20	36.16	35.49	35.55	42.19	42.15
21	18.50	18.93	29.02	26.99	26.73
22	33.95	33.34	33.23	37.74	37.75
23	26.08	23.86	29.50	29.21	29.23
24	45.84	45.14	22.26	16.72	17.69
25	29.16	27.79	16.65	16.78	16.72
26	19.41	19.70	17.73	18.67	17.50
27	19.04	18.61	24.87	24.53	24.20
28	23.07	22.60	177.30	177.01	176.45
29	11.81	11.66	28.94	26.72	27.03
30		100.80	24.65	17.52	17.03
1´		73.45	95.90	95.87	95.89
2´		76.76	74.16	72.66	74.10
3´		70.08	79.00	79.30	79.00
4´		76.91	71.08	71.45	72.67
5´		61.08	79.45	79.00	79.32
6´			62.17	62.36	62.36

位置 Position	化合物6 Compound 6	化合物7 Compound 7	化合物8 Compound 8	化合物9 Compound 9	化合物10 Compound 10	化合物14 Compound 14
1	61.02	60.89	60.85	42.63	42.65	38.21
2	158.17	157.92	156.83	67.29	69.52	27.91
3	131.22	132.11	133.65	80.25	84.53	79.75
4	49.18	49.21	42.01	41.39		39.88
5	63.68	63.84	63.70		56.69	56.80
6	18.05	18.11	17.65	25.05	19.68	19.54
7	35.10	34.96	34.57	34.20	34.10	34.06
8	42.01	41.61	42.37	39.51		40.60
9	43.93	44.45	42.48		43.09
10	50.92	51.11	50.94	39.61	39.21
11	27.20	27.11	26.94	27.44	24.74	24.10
12	128.13	132.11	128.15	129.48	129.25	123.68
13	140.25	145.16	140.26	140.23	140.12	145.26
14	42.39	42.43	42.37	42.87	41.10	42.93
15	29.71	29.57	29.69	29.72	29.30	28.78
16	26.39	28.39	27.05	26.74	26.60	24.56
17	48.32	46.11	48.30	48.35	48.17	47.69
18	54.81	45.16	54.80	55.23	55.09	42.78
19	72.66	81.11	72.64	73.73	73.58	47.30
20	42.35	35.72	43.68	43.23	40.51	31.66
21	27.16	29.15	26.38	19.42	27.06	34.90
22	38.52	33.70	38.51	39.17	39.02	33.87
23	25.14	24.77	30.12	29.38	29.58	28.88
24	66.52	66.50	21.77	22.58	16.60	16.35
25	19.58	19.10	18.96	17.68	17.05	15.92
26	18.81	19.45	18.84	16.75	17.50	17.76
27	25.42	25.70	25.42	27.20	24.81	26.43
28	180.67	180.94	180.66	182.49	182.34	181.93
29	26.95	28.89	27.14	17.01	27.29	33.60
30	16.78	16.78	16.76	24.84	17.45	24.02

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