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Bulletin of Botanical Research ›› 2018, Vol. 38 ›› Issue (4): 611-618.doi: 10.7525/j.issn.1673-5102.2018.04.017

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Effect of Farnesyl-pyrophosphate Synthase(FPS) on the Biosynthesis of Saponins in Panax japonicus

LIU Mei-Jia, YU Yi-Lin, JIANG Sen, ZHANG Xiang, LIU Di-Qiu, GE Feng   

  1. Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500
  • Received:2018-02-28 Online:2018-07-15 Published:2018-07-21
  • Supported by:
    National Natural Science Foundation of China(81560616)

Abstract: Panax japonicus recognized as a valuable Chinese herb has been used for thousands of years. P.japonicus saponins are the main bioactive ingredients composed by dammarane-type and oleanane-type triterpenoid saponins. So far, because of the lack of study about the biosynthesis of P.japonicus saponins, it's necessary to carry out the basic research on saponins biosynthesis. Farnesyl pyrophosphate synthase(FPS) may be a key enzyme in the synthetic pathway of saponins in plants. The cDNA sequence of PjFPS was cloned and bioinformatics analysis was conducted. Overexpression vector pCAMBIA2300s-PjFPS was constructed, and then transferred into the P.japonicus cells to obtain transgenic cell lines successfully. The relative expression levels of PjFPS, the enzyme activities of PjFPS, the contents of PJS and phytosterols in transgenic cell lines were determined. All of them in PjFPS transgenic cells achieved enhancements compared with the control. Furthermore, the expressions of key enzyme genes were promoted because the metabolic flux of saponins was increased. Especially, the expression level of PjFPS, the enzyme activity and total P.japonicus saponins content of the best-performing positive cell line were 12, 4 and 3 times of those in control, respectively. Meanwhile, the content of phytosterol in transgenic cell lines was also enhanced. This study realized the biosynthetic regulation of saponins by controlling the expression of a key enzyme gene, and thus to provide theoretical reference and basis for acquiring an efficient and stable technique of regulating the P.japonicus saponins biosynthesis.

Key words: Panax japonicus, Panax, ginsenoside, triterpenoid, overexpression

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