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Bulletin of Botanical Research ›› 2020, Vol. 40 ›› Issue (3): 468-475.doi: 10.7525/j.issn.1673-5102.2020.03.019

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An Efficient Method for Separation and Purification of Betulin from Birch Bark

LI Ying1, LI Lin-Xi1, ZHANG Yu-Qi1, WANG Yu1, ZHAN Ya-Guang1,2, YIN Jing1,2   

  1. 1. Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin 150040;
    2. State Key Laboratory of Tree Genetic Breeding, Northeast Forestry University, Harbin 150040
  • Received:2019-10-09 Online:2020-05-05 Published:2020-05-29
  • Supported by:
    National Natural Science Foundation of China(31570589);Northeast Forestry University Education and Teaching Reform Project(DGY2017-34);Central University Basic Research Business Expenses Special Fund Project(257-2017DY02)

Abstract: The experiment was conducted to obtain a simple and efficient method for extracting and purifying betulin from the bark of birch by comparing different methods. By comparing three extraction schemes of betulin by different extraction time, ultrasonic oscillation method and rotary evaporator recovery. And methanol-chloroform recrystallization method, absolute alcohol recrystallization method and macroporous resin chromatography were further used to purify the initial extract of betulin. The best extraction and purification scheme of betulin was obtained. The birch bark samples were soaked in 95% ethanol solution with a liquid-solid ratio of 50:1(mL:g) for 120 h. The filtrate was filtered by filter paper. The filtrate was heated and refluxed at 50℃ for 5 h. The white solid powder was obtained by vacuum distillation. Then 70 mL ethyl acetate was used as solvent, and the solid was heated and refluxed for 90 min, then filtered. The filtrate is concentrated to dry at 50℃ in a crucible. Then recrystallize with anhydrous ethanol as solvent. Anhydrous ethanol:solid=30 mL:1 g, recrystallize at -20℃. The recrystallization yield can reach 38.00%, and the highest purity can reach 99.80%. The optimized extraction and purification method of betulin is simple, high yield and safe reagent. It is an effective method for large-scale extraction of betulin.

Key words: birch, betulin, separation, purification

CLC Number: