Effects of Cycle Rejuvenation on Growth, Reproduction and Physiology of Fraxinus mandshurica

doi:10.7525/j.issn.1673-5102.2024.05.009

Abstract

Abstract:

To address the problem of the rooting ability of Fraxinus mandshurica cuttings significantly decreasing after three years due to age effect， four-year-old F. mandshurica seedlings were used to investigate the role of cycle rejuvenation through cuttings in overcoming age effect in this study. The impact of the number of cycles and topping treatment on the sprouting， growth， and rooting of F. mandshurica cuttings， as well as the physiological changes during its rejuvenation process were examined. The results showed that the measurement of increasing the number of rejuvenation cycles and topping treatment both enhanced the degree of rejuvenation in F. mandshurica， which were characterized by an increased number of sprouted branches， finer branches， and shorter internodes， while significantly improved the regeneration ability of the cuttings. When the number of rejuvenation cycles reached three and was combined with topping treatment， the number of sprouted branches increased by 6.56 times， and the rooting rate increased by 7.44 times compared to non-rejuvenated treatment. During one to three rejuvenation cycles， the indole acetic acid（IAA） and cytokinin（CTK） levels in the plants increased， the gibberellin（GA₃） levels significantly increased only at the third cycle， and the abscisic acid （ABA） levels decreased gradually. After rejuvenation， the activities of catalase（CAT）， peroxidase（POD）， and superoxide dismutase（SOD） and the contents of malondialdehyde（MDA） and soluble sugars decreased in the regenerated plants. These physiological changes were consistent with the juvenile state of the plants. Cycle rejuvenation can effectively restore the juvenile state， induce the formation of sprouts with higher IAA and GA₃ levels and lower ABA levels， and simultaneously reduce the oxidative state and soluble sugar content in F. mandshurica. These changes are beneficial for achieving rejuvenation of F. mandshurica， thereby increasing the rooting rate of F. mandshurica cuttings and enabling rapid and efficient clonal propagation of superior F. mandshurica varieties.

Key words: Fraxinus mandshurica, rejuvenation, cutting propagation, hormones, antioxidant enzyme activity, soluble sugar content

CLC Number:

Q945.4

Guangzhou PENG, Jianfei LIU, Qiaoxin WANG, Kezhuo FU, Ye ZHANG, Chenxi ZHANG, Yaguang ZHAN. Effects of Cycle Rejuvenation on Growth, Reproduction and Physiology of Fraxinus mandshurica[J]. Bulletin of Botanical Research, 2024, 44(5): 721-729.

Figures/Tables 6

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