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Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (4): 557-563.doi: 10.7525/j.issn.1673-5102.2021.04.011

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Callus Induction and Somatic Embryogenesis and Physiological State Analysis from Mature Zygotic Embryo Explant of Syringa reticulata var. mandshurica

Chun-Xue PENG, Xue-Mei CUI, Hai-Long SHEN()   

  1. State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040
  • Received:2020-05-18 Online:2021-07-20 Published:2021-03-24
  • Contact: Hai-Long SHEN E-mail:shenhl-cf@nefu.edu.cn
  • About author:PENG Chun-Xue(1992—),female,master,mainly engaged in research of tree tissue culture and plant propagation biology and technology.
  • Supported by:
    National Natural Science Foundation of China(31570596)


In order to optimize somatic embryo induction system of Syringa reticulata var. mandshurica, callus induction and somatic embryogenesis from mature zygotic embryo were conducted by adjusting the type and concentration of plant growth regulators. The morphological observation and physiological analysis of the explants in the culture process were carried out. The results showed that: ①Somatic embryo regenerate from mature zygotic embryo explant 30 d cultured later and cotyledonous somatic embryo can be obtained cultured 60 d later; ②BA played a leading role in callus induction, the callus induction rate was reached 100% in the combinations of 0.5 mg·mL-1 BA with 5-6 mg·mL-1 NAA, and the somatic embryo induction rate reached 8% in the combination of 0.5 mg·mL-1 BA and 5 mg·mL-1 NAA; ③Polyphenol content increased sharply at the primary stage of callus formation and kept a relatively high level in the process of culture. The PAL and POD activity increased and the MDA and SOD activity decreased in cotyledonous stage of somatic embryo.

Key words: Syringa reticulata var. mandshurica, somatic embryogenesis, plant growth regulators, antioxidant enzyme activity

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