Table 1

HB63 gene cloning and quantitative analysis primers and vector primers

Fig.1

PCR production of PsnHB63

Fig.2

Amino acid content of HB63 protein

Fig.3

PsnHB63 protein signal domain，transmembrane domain and prophecyA.Prediction of protein signal peptide of PsnHB63 gene；B.Predictionand analysis of transmembrane structure of PsnHB63 gene protein；C.Distribution map of the amino acid hydrophobicity region of PsnHB63 gene protein

Fig.4

PsnHB63 protein conserved domain

Fig.5

PsnHB63 protein secondary and tertiary structure measurementA.Prediction of the tertiary structure of PsnHB63 gene protein；B.Prediction of the secondary structure of PsnHB63 gene protein

Fig.6

Homologous sequence alignment of PsnHB63

Fig.7

Phylogenetic Tree of PsnHB63

Fig.8

Subcellularlocalization of HB63 proteinA，D.Were observed in dark filed；B，E.Were observed in bright field；C，F.Were observed in combination

Fig.9

Changes of relative expression levels of HB63 gene in different parts of P. nigra under salt stress at different times

Fig.10

Changes in the relative expression of HB63 gene of P. nigra with mannitol at different times and different parts under simulated drought